Protein Info for EX28DRAFT_0821 in Enterobacter asburiae PDN3

Annotation: GTP-binding protein YchF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00092: GTP-binding protein YchF
  • amino acids 1 to 364 (364 residues), 580.3 bits, E=8.3e-179
• PF02421: FeoB_N
  • amino acids 5 to 45 (41 residues), 32.8 bits, 7.2e-12
• PF01926: MMR_HSR1
  • amino acids 5 to 112 (108 residues), 72.5 bits, E=4.7e-24
• PF06071: YchF-GTPase_C
  • amino acids 280 to 363 (84 residues), 133 bits, E=5.1e-43

Best Hits

Swiss-Prot: 94% identical to YCHF_SHIFL: Ribosome-binding ATPase YchF (ychF) from Shigella flexneri

KEGG orthology group: K06942, (no description) (inferred from 99% identity to kpe:KPK_2060)

MetaCyc: 94% identical to redox-responsive ATPase YchF (Escherichia coli K-12 substr. MG1655)
Nucleoside-triphosphatase. [EC: 3.6.1.15, 3.6.1.5]

Predicted SEED Role

"GTP-binding and nucleic acid-binding protein YchF" in subsystem Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5

Protein Sequence (364 amino acids)

>EX28DRAFT_0821 GTP-binding protein YchF (Enterobacter asburiae PDN3)
MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDPRLDQLAEIVKP
QRILPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGHVVRCFENDNIIHVNNKVD
PADDIDVINTELALSDLDTCERAIHRVQKKAKGGDKDAKAELAALEKCLPQLENAGMLRA
LKNLTEEDKAAIKYLSFLTLKPTMYIANVNEDGFENNPYLDKVREIAAAEGSVVVAVCAA
VESDIAELDDADREEFMAELGLEEPGLNRVIRAGYELLNLQTYFTAGVKEVRAWTIPVGA
TAPQAAGKIHTDFEKGFIRAQTIAFEDFITYKGEQGAKEAGKMRAEGKDYIVKDGDVMNF
LFNV