Protein Info for EX28DRAFT_0694 in Enterobacter asburiae PDN3

Annotation: Holliday junction endonuclease RuvC (EC 3.1.22.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02075: RuvC
  • amino acids 4 to 150 (147 residues), 228.9 bits, E=1.1e-72
• TIGR00228: crossover junction endodeoxyribonuclease RuvC
  • amino acids 4 to 159 (156 residues), 288.5 bits, E=6e-91

Best Hits

Swiss-Prot: 99% identical to RUVC_CITK8: Crossover junction endodeoxyribonuclease RuvC (ruvC) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K01159, crossover junction endodeoxyribonuclease RuvC [EC: 3.1.22.4] (inferred from 98% identity to eoj:ECO26_2715)

MetaCyc: 98% identical to crossover junction endodeoxyribonuclease RuvC (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4)" in subsystem DNA-replication or RuvABC plus a hypothetical (EC 3.1.22.4)

Isozymes

Compare fitness of predicted isozymes for: 3.1.22.4

Use Curated BLAST to search for 3.1.21.10 or 3.1.22.4

Protein Sequence (173 amino acids)

>EX28DRAFT_0694 Holliday junction endonuclease RuvC (EC 3.1.22.4) (Enterobacter asburiae PDN3)
MSIILGIDPGSRVTGYGVIRQVGRQLTYLGSGCIRTKVDDLPSRLKLIYAGVSEIITQFQ
PDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQDLPVFEYAARQVKQTVVGIGSAEKS
QVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQMSESRLNLARGRLR