Protein Info for EX28DRAFT_0662 in Enterobacter asburiae PDN3

Annotation: Chemotaxis protein histidine kinase and related kinases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 678 PF01627: Hpt" amino acids 8 to 97 (90 residues), 70.1 bits, see alignment E=4e-23 PF09078: CheY-binding" amino acids 166 to 228 (63 residues), 64 bits, see alignment E=2.8e-21 PF02895: H-kinase_dim" amino acids 286 to 348 (63 residues), 73.2 bits, see alignment E=4.8e-24 PF02518: HATPase_c" amino acids 396 to 532 (137 residues), 66 bits, see alignment E=1e-21 PF01584: CheW" amino acids 538 to 664 (127 residues), 103.8 bits, see alignment E=1.5e-33

Best Hits

Swiss-Prot: 87% identical to CHEA_SALTY: Chemotaxis protein CheA (cheA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 98% identity to enc:ECL_01404)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (678 amino acids)

>EX28DRAFT_0662 Chemotaxis protein histidine kinase and related kinases (Enterobacter asburiae PDN3)
MSMDISDFYQTFFDEADELLADMEQHLLDLVPEAPDSEQLNAIFRAAHSIKGGAGTFGFT
ILQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQLDAYKSSAEPDAASFEYIC
NALRQLALEAKGEVAAAVVPAAKLSVVDAVAADEAAPAAAQAGKLRVVLSRLKESEVNLL
EEELGNLAKLSNVVKGKDSLAATLDDGIGQDDIVAVLCFVIEADQIAFETETAAVDAPAA
AEEVAVVAQAAAPAVAPAAPALKAVPKEAAAPGRGEKPAARSSESTSIRVAVEKVDQLIN
LVGELVITQSMLAQRSNELDPVTHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFSRF
PRLVRDLASKLNKQIELTLMGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPENRIAAG
KSPVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAISQGMAVNENMTDEEVGMLIFA
PGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGSGTTIRILLPLTLAILDGMS
VKVADEVFILPLNAVMESLQPREEDLHPLAGGERVLEVRGEYLPLVELWKVFEVDGAKTE
ATQGIVVILQSAGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAATILGDGSVALIVD
VSALQGLNREQRVAYTAA