Protein Info for EX28DRAFT_0656 in Enterobacter asburiae PDN3

Annotation: alpha,alpha-trehalose-phosphate synthase [UDP-forming]

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 474 transmembrane" amino acids 21 to 42 (22 residues), see Phobius details TIGR02400: alpha,alpha-trehalose-phosphate synthase (UDP-forming)" amino acids 3 to 452 (450 residues), 609.4 bits, see alignment E=1.9e-187 PF00982: Glyco_transf_20" amino acids 17 to 452 (436 residues), 481.8 bits, see alignment E=1.2e-148

Best Hits

Swiss-Prot: 88% identical to OTSA_ENT38: Trehalose-6-phosphate synthase (otsA) from Enterobacter sp. (strain 638)

KEGG orthology group: K00697, alpha,alpha-trehalose-phosphate synthase (UDP-forming) [EC: 2.4.1.15] (inferred from 98% identity to enc:ECL_01398)

MetaCyc: 82% identical to trehalose-6-phosphate synthase (Escherichia coli K-12 substr. MG1655)
Alpha,alpha-trehalose-phosphate synthase (UDP-forming). [EC: 2.4.1.15]

Predicted SEED Role

"Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15)" in subsystem Trehalose Biosynthesis (EC 2.4.1.15)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (474 amino acids)

>EX28DRAFT_0656 alpha,alpha-trehalose-phosphate synthase [UDP-forming] (Enterobacter asburiae PDN3)
MGRLVVVSNRIAPPDDKKASAGGLAVGVLGALKAAGGLWFGWSGEISEEEKPLKKVTRGN
ITWASFALKEKDYDEYYSQFSNAVLWPAFHYRLDLVKFQRESFEGYSRVNALLADKLLPL
IEEDDILWIHDYHLLPFARELRKRGVNNRIGFFLHIPFPTPEIFTALPPHEELLEALCDY
DLLGFQTENDRLAFLDSVSGKARLVTKGGKTHTAWGKTFHTEVYPIGIEPDEIAEQASGP
LPPKLAQLKNELKHVKNIFSVERLDYSKGLPERFLAYETLLDKYPQHHGKIRYTQIAPTS
RGEVQAYQDIRHQLETEAGRINGRYGQLGWTPLFYLNQHFDRKILMKVFRYADVGLVTPL
RDGMNLVAKEYVAAQDPADPGVLVLSQFAGAANELTSALIVNPYDRDDVANALNRALTMP
LTERISRHADMMETIRKNDINHWQARFIQDLRDIAPQSHEGDLQKKIATFPKLA