Protein Info for EX28DRAFT_0643 in Enterobacter asburiae PDN3

Annotation: ABC-type branched-chain amino acid transport systems, periplasmic component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 373 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF13458: Peripla_BP_6" amino acids 28 to 368 (341 residues), 218.1 bits, see alignment E=4.4e-68 PF13433: Peripla_BP_5" amino acids 30 to 370 (341 residues), 73.7 bits, see alignment E=2.3e-24 PF01094: ANF_receptor" amino acids 51 to 365 (315 residues), 95.9 bits, see alignment E=3.8e-31

Best Hits

Swiss-Prot: 48% identical to LIVB1_BRUSU: Leu/Ile/Val-binding protein homolog 1 (BR1785) from Brucella suis biovar 1 (strain 1330)

KEGG orthology group: K01999, branched-chain amino acid transport system substrate-binding protein (inferred from 99% identity to enc:ECL_01382)

Predicted SEED Role

"Branched-chain amino acid ABC transporter, amino acid-binding protein (TC 3.A.1.4.1)" in subsystem ABC transporter branched-chain amino acid (TC 3.A.1.4.1) (TC 3.A.1.4.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (373 amino acids)

>EX28DRAFT_0643 ABC-type branched-chain amino acid transport systems, periplasmic component (Enterobacter asburiae PDN3)
MSLKFIRTPLSLVLAGCLVTAFSARADIVIGVAGPFTGPNATYGDQYWHGATQAAEDINA
AGGINGEKIKLVQGDDACEPKQAVAVANRLVDQDKVNAVVGHFCSSSTMPASEVYSDAGI
IAITPGSTNPLITERGMSDMFRMCGRDDQQGQVASDFIIDKLKAKRVVIIHDKDTYGQGL
ADATKAALAKRGVQDVMYEGLSRGEKDFNALVTKIGAQKPDVVFFGGCHPEAGPLVRQMR
EQGVQAKFFSGDCIVNEEMVTAAGGPQYTNGIYMTFGKDPRLIPDGKAVIEKFRAGKFEP
EGYTLYSYASVQAIAAAFKATGGKDSAKASEWLKANSVDTVMGKKAWDSKGDLKVSDYVV
YQWDDKGKYKEVQ