Protein Info for EX28DRAFT_0616 in Enterobacter asburiae PDN3

Annotation: flagellar basal-body M-ring protein/flagellar hook-basal body protein (fliF)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 558 transmembrane" amino acids 26 to 46 (21 residues), see Phobius details amino acids 456 to 474 (19 residues), see Phobius details TIGR00206: flagellar M-ring protein FliF" amino acids 9 to 557 (549 residues), 711.2 bits, see alignment E=5.7e-218 PF01514: YscJ_FliF" amino acids 47 to 221 (175 residues), 236.8 bits, see alignment E=1.5e-74 PF08345: YscJ_FliF_C" amino acids 253 to 435 (183 residues), 156.6 bits, see alignment E=6.5e-50

Best Hits

Swiss-Prot: 85% identical to FLIF_SALTY: Flagellar M-ring protein (fliF) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02409, flagellar M-ring protein FliF (inferred from 96% identity to enc:ECL_03220)

Predicted SEED Role

"Flagellar M-ring protein FliF" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (558 amino acids)

>EX28DRAFT_0616 flagellar basal-body M-ring protein/flagellar hook-basal body protein (fliF) (Enterobacter asburiae PDN3)
MSATASTAPQNKSLEWMNRLRANPKIPLIVAGAAAIAILVAMVLWAKSPDYRTLYSNLSD
QDGGAIVTQLTQMNIPYRFADNGGALEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEK
FGISQFSEQVNYQRALEGELARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVNL
EPGRALDEGQISAVTHLVSSAVAGLPPGNVTLVDQSGHLLTQSNTAGRDLNDAQLKYAAD
VENRLQRRIEAILGPVVGNSNVHAQVTAQIDFANKEQTEEQYSPNGDAAQAVMRSRQINS
NEQVGGAYPGGVPGALSNQPAPANAAPISTPPANQQNGQQNQQTTSTTSNAGPRTSSRNE
TTNYEVDRTIRHTKLNVGDIQRLSVAVVVNYKTLADGKPLPLTAEQMKQIENLTREAMGY
SEKRGDTLNVVNSPFNSVEETGGELPFWQQQAFIDQLMSAGRWLLVLIVAWLLWRKGVRP
QLQRRAEAEKAAREQMNTRQETEEAVEVRLSKDEQMQQRRANQRMGAEVMSQRIREMSDN
DPRVVALVIRQWMGNEHE