Protein Info for EX28DRAFT_0589 in Enterobacter asburiae PDN3

Annotation: Signal transduction histidine kinase regulating citrate/malate metabolism

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 538 transmembrane" amino acids 20 to 41 (22 residues), see Phobius details amino acids 181 to 202 (22 residues), see Phobius details PF17203: sCache_3_2" amino acids 46 to 177 (132 residues), 55 bits, see alignment E=2.5e-18 PF14689: SPOB_a" amino acids 332 to 374 (43 residues), 38.9 bits, see alignment 1.3e-13 PF02518: HATPase_c" amino acids 430 to 531 (102 residues), 77.2 bits, see alignment E=3.2e-25 PF14501: HATPase_c_5" amino acids 430 to 524 (95 residues), 34.4 bits, see alignment E=4.6e-12

Best Hits

KEGG orthology group: K07701, two-component system, CitB family, sensor histidine kinase DcuS [EC: 2.7.13.3] (inferred from 96% identity to enc:ECL_03247)

Predicted SEED Role

"Fumarate respiration sensor kinase protein DcuS"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (538 amino acids)

>EX28DRAFT_0589 Signal transduction histidine kinase regulating citrate/malate metabolism (Enterobacter asburiae PDN3)
MSDLQPFLTTRKRPMKLNTLVTLMVCAIIASVLLVVFALYSVQITRATRDDVKDTALGIA
RTLANSPEIQRGLMQPPQADIIQPIAKAVTQRNDLLLTVVTDMRGIRYSHPNEALIGLHF
IGNDLEPALEGKENVSVNRGALAEALRVFTPVYDSQHDQIGVVVVGISLKKVEDQIARGR
LNAVWTILFSIFMSSMAIWGLVRVLKRILFGLEPYEISALFEQRQAMLQSLREGVLAVDI
HGRVTMINQTAREILLLPSGKQTENASAPLLASLREVSKTGVARQDQEISCNGRLLLCNM
VPVKSQDRVIGAITTFRDKTEISQLMQRIDGMVNYVDALRSHTHEFMNKLHVILGLLHMK
RYDKLEEYIIQTAHNYQTDIGTIQSKVKSPVIAGFLLGKINRAKEAGVTLTLADECQIPD
TASEEQVAVLITVLGNLIENALDAMEGQQEGEIGLLLHYQNGWLSCEVSDDGPGIDPAQL
EAIFTKGFSTKGENRGVGLFLARQQIQNLGGDITVESEPGVFTQFFVHIPWDSERNIA