Protein Info for EX28DRAFT_0586 in Enterobacter asburiae PDN3

Annotation: flavocytochrome c

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 926 PF00724: Oxidored_FMN" amino acids 8 to 340 (333 residues), 246.7 bits, see alignment E=1.9e-76 PF04205: FMN_bind" amino acids 399 to 461 (63 residues), 23.5 bits, see alignment 3.2e-08 PF01266: DAO" amino acids 483 to 597 (115 residues), 31.9 bits, see alignment E=4.9e-11 PF00890: FAD_binding_2" amino acids 483 to 905 (423 residues), 276.5 bits, see alignment E=2.1e-85 TIGR01813: flavocytochrome c" amino acids 483 to 916 (434 residues), 502.5 bits, see alignment E=4.9e-155 PF12831: FAD_oxidored" amino acids 483 to 668 (186 residues), 57 bits, see alignment E=1e-18 PF07992: Pyr_redox_2" amino acids 483 to 672 (190 residues), 45.2 bits, see alignment E=3.9e-15 PF03486: HI0933_like" amino acids 483 to 672 (190 residues), 38.3 bits, see alignment E=3.2e-13 PF13450: NAD_binding_8" amino acids 486 to 525 (40 residues), 28.5 bits, see alignment (E = 6.6e-10)

Best Hits

KEGG orthology group: K00244, fumarate reductase flavoprotein subunit [EC: 1.3.99.1] (inferred from 97% identity to enc:ECL_03250)

MetaCyc: 87% identical to fumarate reductase (NADH) (Klebsiella pneumoniae)
Fumarate reductase (NADH). [EC: 1.3.1.6]

Predicted SEED Role

"Fumarate reductase flavoprotein subunit (EC 1.3.99.1)" in subsystem Succinate dehydrogenase (EC 1.3.99.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.1

Use Curated BLAST to search for 1.3.1.6 or 1.3.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (926 amino acids)

>EX28DRAFT_0586 flavocytochrome c (Enterobacter asburiae PDN3)
MSTNERILSPFTLPNGTELKNRLLMAPMTTCTGYFDGTVTSELVEYYRARSGSIGTIIVE
CCFVDDLGLAFPGAIGIDNDEKIAGLAKIAEAIKSKGSKALLQIYHGGRMVDPKLIGGRT
PVGPSAVAAPREGAATPVALTAEEVEGMIGKFGEAVCRAIQAGFDGVEIHGANTYLIQQF
YSPNSNQRDDEWGGSRDNRAKFPLAVLDITHKMVRQYADDAFIIGYRFSPEEMEVPGIRF
DDTMYLLEKLAARGVDYLHFSVGATLRPSIVDTQDPTPLIEKYCAMRSETLAQVPVMGVG
GVVNASDVNEALDHGYDLIAVGRATIAYPDWTDRIASGESLELFMDSTQREALSIPEPLW
RFSLVEAMIRDMSMGESKFKPGTFAEKVQDDANELVINVSLETDRIADIELASGPSEDVE
FVTSFEEIRTRILDANTPHVDAITGATSQSEAVKKAVSKAMLKSSKALAAEEGVDPNETR
SVDVVVVGSGGAGLAAAIQAHDEGASVLIVEKMPTIGGNTIKASAGMNAAETRFQRVKGI
QDSKELFYQESLKGGGNKNNPELLRRFVENAPQAIEWLATRGIMLNDITTTGGMSIDRTH
RPKDGSAVGGYLISGLVRNVNKRNIEVMLDTSVSDILFENGEVTGVRLTTEENETVTVAT
KSVIVATGGFSANSQMVVKYRPDLEGFVTTNHKGATGGGIALLERIGAGTVDMGEIQIHP
TVEQNTSYLISESIRGGGAILVNQKGERFYNEMSTRDKVSASIIALPEKYAYIVFDEHVR
AKNKAADEYIAKGFVTSASSPKALAEALGMDYHAFLATLERYNGFVEKQHDDDFGRTTAL
RAPINEGPFYAIQIAPGVHHTMGGVTINTETCVLDSNHNVLPGAFAAGEVVGGIHGGNRI
GGNAVADIIIFGTLAGHQAAMRSKKQ