Protein Info for EX28DRAFT_0556 in Enterobacter asburiae PDN3

Annotation: metabolite-proton symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 438 transmembrane" amino acids 25 to 48 (24 residues), see Phobius details amino acids 61 to 84 (24 residues), see Phobius details amino acids 96 to 117 (22 residues), see Phobius details amino acids 120 to 149 (30 residues), see Phobius details amino acids 161 to 184 (24 residues), see Phobius details amino acids 196 to 215 (20 residues), see Phobius details amino acids 255 to 275 (21 residues), see Phobius details amino acids 288 to 310 (23 residues), see Phobius details amino acids 318 to 336 (19 residues), see Phobius details amino acids 342 to 363 (22 residues), see Phobius details amino acids 383 to 406 (24 residues), see Phobius details amino acids 413 to 432 (20 residues), see Phobius details PF00083: Sugar_tr" amino acids 27 to 436 (410 residues), 80.7 bits, see alignment E=1.1e-26 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 30 to 425 (396 residues), 496.4 bits, see alignment E=3.2e-153 PF07690: MFS_1" amino acids 57 to 299 (243 residues), 62.7 bits, see alignment E=3e-21 amino acids 265 to 432 (168 residues), 45.8 bits, see alignment E=4.2e-16

Best Hits

Swiss-Prot: 91% identical to SHIA_ECOLI: Shikimate transporter (shiA) from Escherichia coli (strain K12)

KEGG orthology group: K08172, MFS transporter, MHS family, shikimate and dehydroshikimate transport protein (inferred from 95% identity to enc:ECL_03326)

MetaCyc: 91% identical to shikimate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-27

Predicted SEED Role

"Shikimate transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (438 amino acids)

>EX28DRAFT_0556 metabolite-proton symporter (Enterobacter asburiae PDN3)
MDSTLISDRPNEETPSLNRARRAALGSFAGAVVDWYDFLLYGITAALVFNREFFPQVSPA
MGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVWMMGIATALIGILPSFASI
GWWAPVLLVTLRAIQGFAVGGEWGGAALLSVESAPKHRKAFYSSGVQVGYGVGLLLSTGL
VSLISQLTTDEQFLSWGWRIPFIFSIVLVIAALWIRNGMEESAEFEKQQLEKPLAKKRLP
VMEALVQHPGAFLKIIALRLCELLTMYIVTAFALNYSTQNLGLPRELFLNIGLLVGGISC
LTIPCFAWLADRFGRRRVYITGALIGTLSAWPFFMALEAQSIFWIVFFAIMLANIAHDMV
VCVQQPMFTELFGASYRYSGAGVGYQVASVVGGGFTPFIAAALVTFSGGNWHSVAIYLLA
GCLLSAATALLMKETSHG