Protein Info for EX28DRAFT_0539 in Enterobacter asburiae PDN3

Annotation: histidinol dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 PF00815: Histidinol_dh" amino acids 28 to 427 (400 residues), 544.8 bits, see alignment E=7.3e-168 TIGR00069: histidinol dehydrogenase" amino acids 36 to 426 (391 residues), 530.6 bits, see alignment E=1.4e-163

Best Hits

Swiss-Prot: 88% identical to HISX_SHISS: Histidinol dehydrogenase (hisD) from Shigella sonnei (strain Ss046)

KEGG orthology group: K00013, histidinol dehydrogenase [EC: 1.1.1.23] (inferred from 95% identity to enc:ECL_03340)

MetaCyc: 88% identical to histidinal/histidinol dehydrogenase (Escherichia coli K-12 substr. MG1655)
Histidinol dehydrogenase. [EC: 1.1.1.23]

Predicted SEED Role

"Histidinol dehydrogenase (EC 1.1.1.23)" in subsystem Histidine Biosynthesis (EC 1.1.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (434 amino acids)

>EX28DRAFT_0539 histidinol dehydrogenase (Enterobacter asburiae PDN3)
MSFNTIIDWNTCSDAQQRELLMRPAISASESISRTVAEILDNVKARGDDALREYSAKFDK
TEVGALRVTEQEIIDASNRLGDDIKQAMSVAVKNIDTFHTAQKLQTVDVETLPGVRCQQV
TRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCQKVVLCSPPPIADEILYAAKLCGVQA
VYKVGGAQAISALAFGTESIPKVDKIFGPGNAYVTEAKRQVSQRLDGAAIDMPAGPSEVL
VIADSGATPDFVASDLLSQAEHGPDSQVILLTPDADMAKRVGEAVERQLADLPRADTARQ
ALAASRLIVARDLAQCIAISNQYGPEHLIIQTRSARDLVDSITSAGSVFLGDWSPESAGD
YASGTNHVLPTYGYTSTCSSLGLADFQKRMTVQELSREGFASLASTIETLAAAERLTAHK
NAVTLRVAALKEQA