Protein Info for EX28DRAFT_0533 in Enterobacter asburiae PDN3

Annotation: phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)/phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01502: PRA-CH
  • amino acids 33 to 105 (73 residues), 111.4 bits, E=1.5e-36
• TIGR03188: phosphoribosyl-ATP diphosphatase
  • amino acids 115 to 198 (84 residues), 102.4 bits, E=5.9e-34
• PF01503: PRA-PH
  • amino acids 115 to 202 (88 residues), 79.7 bits, E=1.6e-26

Best Hits

Swiss-Prot: 90% identical to HIS2_SHISS: Histidine biosynthesis bifunctional protein HisIE (hisI) from Shigella sonnei (strain Ss046)

KEGG orthology group: K11755, phosphoribosyl-ATP pyrophosphohydrolase / phosphoribosyl-AMP cyclohydrolase [EC: 3.5.4.19 3.6.1.31] (inferred from 96% identity to enc:ECL_03346)

MetaCyc: 90% identical to putative bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase (Escherichia coli K-12 substr. MG1655)
Phosphoribosyl-ATP diphosphatase. [EC: 3.6.1.31]; Phosphoribosyl-AMP cyclohydrolase. [EC: 3.6.1.31, 3.5.4.19]

Predicted SEED Role

"Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) / Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31)" in subsystem Histidine Biosynthesis (EC 3.5.4.19, EC 3.6.1.31)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• L-histidine biosynthesis (10/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Histidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.19 or 3.6.1.31

Protein Sequence (203 amino acids)

>EX28DRAFT_0533 phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)/phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (Enterobacter asburiae PDN3)
MLTEQQQAQLDWEKTDGLLPVVVQHAVSGEVLMLGYMNQDALTKTIDSGKVTFYSRTKQR
LWTKGETSGHFLNVVSMAPDCDNDTLLVLANPIGPTCHKGTSSCFGETSHQWLFLYQLEQ
LLAERKTADPESSYTAKLYASGTKRIAQKVGEEGVETALAATVHDREELTNEASDLMYHL
LVLLQDQELDLTAVIENLRKRHK