Protein Info for EX28DRAFT_0519 in Enterobacter asburiae PDN3

Annotation: Predicted nucleoside-diphosphate sugar epimerases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 628 transmembrane" amino acids 15 to 35 (21 residues), see Phobius details amino acids 48 to 68 (21 residues), see Phobius details amino acids 80 to 102 (23 residues), see Phobius details amino acids 112 to 129 (18 residues), see Phobius details PF13727: CoA_binding_3" amino acids 67 to 235 (169 residues), 32.3 bits, see alignment E=4e-11 PF04321: RmlD_sub_bind" amino acids 279 to 409 (131 residues), 31.1 bits, see alignment E=5.2e-11 PF02719: Polysacc_synt_2" amino acids 280 to 572 (293 residues), 411.5 bits, see alignment E=7.6e-127 PF01370: Epimerase" amino acids 280 to 496 (217 residues), 58.5 bits, see alignment E=2.8e-19 PF16363: GDP_Man_Dehyd" amino acids 281 to 408 (128 residues), 39.5 bits, see alignment E=2e-13 PF01073: 3Beta_HSD" amino acids 281 to 414 (134 residues), 31 bits, see alignment E=5.7e-11

Best Hits

KEGG orthology group: None (inferred from 63% identity to ssn:SSON_P233)

MetaCyc: 63% identical to UDP-N-acetylglucosamine 4,6-dehydratase (configuration-retaining) (Shigella sonnei)
RXN-13252 [EC: 4.2.1.135]

Predicted SEED Role

"Nucleoside-diphosphate sugar epimerase/dehydratase"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.135

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (628 amino acids)

>EX28DRAFT_0519 Predicted nucleoside-diphosphate sugar epimerases (Enterobacter asburiae PDN3)
MLTYLISLPRPIKRIITLSIDVVLLMCAFWFAFWVRIDSMTPLSSVSHWLLLTSIIVITL
AIFVKLGLYRAVIRYITAKILLVVAIGMLISSLLLIIAAFYTNLYLPRTIPFIYFSFGVL
FVAGSRLAMRMLVNRGLKLGTRVIIYGAGASGRQLLPALSQMDEYYPVAFVDDNRSLQGQ
VIHGVSVFSPEKLAWLIEKYEAKKILLAMPSASRARKNEVISTLEGLPCEVLSIPGMVDL
VEGKARIDALRKVVITDLLGRDPVTPIPELISKNIQGKTVMVTGAGGSIGSELCRQIIRN
KPTQLILFELSEFSLYSIDSELRNLILSQSLNVKIYPILGNVQDMAHLERLIKAFSVETI
YHAAAYKHVPLVEFNVIDGVRNNIFGTLCCAQAAENCGVESFVLISTDKAVRPTNTMGAT
KRMAELILQALASESSKTCFSMVRFGNVLGSSGSVVPLFEKQIAAGGPVTLTHPDITRYF
MTIPEAAQLVIQAGAMGSGGDVFVLDMGDPVKIIDLAHRMIALQGLSVRDDKNPYGDIAI
KTTGLRPGEKLYEELLIGDNVSITQHPRIKTANEVMLPWNEMKDIIAELDSSCRNFDYER
VREILLNAPTAFNPTDDICDLLWKEHKS