Protein Info for EX28DRAFT_0508 in Enterobacter asburiae PDN3

Annotation: Undecaprenyl-phosphate glucose phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 464 transmembrane" amino acids 20 to 37 (18 residues), see Phobius details amino acids 46 to 69 (24 residues), see Phobius details amino acids 81 to 103 (23 residues), see Phobius details amino acids 109 to 130 (22 residues), see Phobius details amino acids 279 to 301 (23 residues), see Phobius details TIGR03023: undecaprenyl-phosphate glucose phosphotransferase" amino acids 42 to 464 (423 residues), 548.7 bits, see alignment E=1.5e-168 TIGR03025: exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase" amino acids 45 to 464 (420 residues), 486.3 bits, see alignment E=1.1e-149 PF13727: CoA_binding_3" amino acids 66 to 238 (173 residues), 144.2 bits, see alignment E=4.5e-46 PF02397: Bac_transf" amino acids 274 to 457 (184 residues), 233.5 bits, see alignment E=1.3e-73

Best Hits

Swiss-Prot: 85% identical to WCAJ_ECOLI: UDP-glucose:undecaprenyl-phosphate glucose-1-phosphate transferase (wcaJ) from Escherichia coli (strain K12)

KEGG orthology group: K03606, putative colanic acid biosysnthesis UDP-glucose lipid carrier transferase (inferred from 97% identity to enc:ECL_03370)

MetaCyc: 85% identical to UDP-glucose:undecaprenyl-phosphate glucose-1-phosphate transferase (Escherichia coli K-12 substr. MG1655)
RXN-11791 [EC: 2.7.8.31]

Predicted SEED Role

"Colanic acid biosynthsis UDP-glucose lipid carrier transferase WcaJ" in subsystem Colanic acid biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.8.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (464 amino acids)

>EX28DRAFT_0508 Undecaprenyl-phosphate glucose phosphotransferase (Enterobacter asburiae PDN3)
MTNLKKRERARTNASLISMVQRFSDITIMVGGLWAVCRIGGLPFLYMHLLMALIALVVFQ
MIGGMTDFYRSWRGVKMTTELMLLLQNWTLSLIFSAGLVAFSHDFDNRLVTYLCWYLLTS
VGMVVCRSLIRFGAGWLRNRGYNRRFVAVAGDLPVGKVLLDSFRKEPWLGFEVVGIYHDA
KPGGVPSDWAGNYEQLIEDAKAGKIHNVYIAMQMRDESRIKQLMRELADTTCSVILIPDV
FTFNILHSRIEEVNGVPVVPLYDTPLSGINRVLKRAEDIVLSSLILLLISPVLCCIALAV
KLSSPGPVIFRQTRYGMDGKPIMVWKFRSMKVMENDKVVTQATQNDPRVTRVGNFLRRTS
LDELPQFINVFTGGMSIVGPRPHAVAHNEQYRSLIEGYMLRHKVKPGITGWAQINGWRGE
TDTLEKMEKRIEFDLEYIREWSIWFDIKIVFLTIFKGFVNKAAY