Protein Info for EX28DRAFT_0501 in Enterobacter asburiae PDN3
Annotation: colanic acid biosynthesis acetyltransferase WcaF
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 88% identical to WCAF_SHIFL: Putative colanic acid biosynthesis acetyltransferase WcaF (wcaF) from Shigella flexneri
KEGG orthology group: K03818, putative colanic acid biosynthesis acetyltransferase WcaF [EC: 2.3.1.-] (inferred from 97% identity to enc:ECL_03377)MetaCyc: 88% identical to colanic acid biosynthesis acetyltransferase WcaF (Escherichia coli K-12 substr. MG1655)
2.3.1.-
Predicted SEED Role
"Colanic acid biosynthesis acetyltransferase WcaF (EC 2.3.1.-)" in subsystem Colanic acid biosynthesis (EC 2.3.1.-)
MetaCyc Pathways
- colanic acid (Escherichia coli K12) biosynthesis (10/11 steps found)
KEGG Metabolic Maps
- 1- and 2-Methylnaphthalene degradation
- Alkaloid biosynthesis I
- Alkaloid biosynthesis II
- Anthocyanin biosynthesis
- Benzoate degradation via CoA ligation
- Biosynthesis of terpenoids and steroids
- Biosynthesis of type II polyketide backbone
- Biosynthesis of unsaturated fatty acids
- Butanoate metabolism
- Carotenoid biosynthesis - General
- Diterpenoid biosynthesis
- Ether lipid metabolism
- Ethylbenzene degradation
- Fatty acid biosynthesis
- Glycerophospholipid metabolism
- Glycosphingolipid biosynthesis - ganglio series
- Histidine metabolism
- Limonene and pinene degradation
- Lipopolysaccharide biosynthesis
- Lysine degradation
- Phenylalanine metabolism
- Tyrosine metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.3.1.-
Use Curated BLAST to search for 2.3.1.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (182 amino acids)
>EX28DRAFT_0501 colanic acid biosynthesis acetyltransferase WcaF (Enterobacter asburiae PDN3) MQDLKGFSVPKGFRGGNGIKVQLWWAVQATLFAWSPQIMYRWRAFLLRLFGAKIGKNVVI RPSVKITYPWKLTLGDYAWVGDDAVLYTLGEITIGANSVVSQKCYLCTGSHDFMSQHFDI TASPIAIGEKCWLATDVFVAPGVSVGDGTVVGARSSVFKSLPANKICRGNPAVVIRERVE TD