Protein Info for EX28DRAFT_0487 in Enterobacter asburiae PDN3

Annotation: PAS domain S-box/diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1109 transmembrane" amino acids 15 to 34 (20 residues), see Phobius details amino acids 39 to 58 (20 residues), see Phobius details amino acids 65 to 85 (21 residues), see Phobius details amino acids 91 to 112 (22 residues), see Phobius details amino acids 124 to 147 (24 residues), see Phobius details amino acids 156 to 177 (22 residues), see Phobius details amino acids 189 to 208 (20 residues), see Phobius details amino acids 212 to 228 (17 residues), see Phobius details amino acids 235 to 256 (22 residues), see Phobius details amino acids 270 to 290 (21 residues), see Phobius details PF05231: MASE1" amino acids 19 to 294 (276 residues), 106.9 bits, see alignment E=4.6e-34 TIGR00229: PAS domain S-box protein" amino acids 299 to 421 (123 residues), 99.5 bits, see alignment E=1.5e-32 amino acids 553 to 676 (124 residues), 41.8 bits, see alignment E=1.1e-14 PF00989: PAS" amino acids 302 to 414 (113 residues), 38.9 bits, see alignment E=3.1e-13 amino acids 560 to 668 (109 residues), 34.7 bits, see alignment E=6.4e-12 PF13426: PAS_9" amino acids 314 to 415 (102 residues), 41.6 bits, see alignment E=5.1e-14 amino acids 566 to 670 (105 residues), 23.9 bits, see alignment E=1.6e-08 PF08447: PAS_3" amino acids 326 to 408 (83 residues), 38.5 bits, see alignment 4.6e-13 amino acids 451 to 537 (87 residues), 31.5 bits, see alignment 6.9e-11 PF08448: PAS_4" amino acids 561 to 672 (112 residues), 29 bits, see alignment E=4.4e-10 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 680 to 842 (163 residues), 126.9 bits, see alignment E=6.6e-41 PF00990: GGDEF" amino acids 682 to 837 (156 residues), 147.8 bits, see alignment E=1e-46 PF00563: EAL" amino acids 894 to 1094 (201 residues), 72.5 bits, see alignment E=1.5e-23

Best Hits

Swiss-Prot: 74% identical to DGCE_ECOLI: Probable diguanylate cyclase DgcE (dgcE) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 92% identity to enc:ECL_03390)

Predicted SEED Role

"FIG00626249: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1109 amino acids)

>EX28DRAFT_0487 PAS domain S-box/diguanylate cyclase (GGDEF) domain (Enterobacter asburiae PDN3)
MNKQYQRVLVTTPHPLIRLVCLGLVTFIFTLFSLELTRFGTLLAPLWFPTAIMMVAFYRH
AGKMWPGIALACSFGNILATWMLFSWGSISLTYTSINVVEAFTGALLLRKLLPWYNPLQN
LNDWVRVAIGSAVIPPLVGGVLVSLLAPGPEPLRNFFVWVLSEAIGALAVVPLGLLFKPH
YLLRHRDPRLLLETLTTLAVTLALSWAAITWLPWPFTCVIVLLMWSAVRLPRMEAFMVFL
VTVMVVSLMIAQNPVALTTQHVGPMLNAPWLPFLMLLLPANVMTMVMYAFRAERKHITES
EERFRNAMEYSAIGMALVSIEGKWLQANKALCNFLGYSQTELQSLTFQQLTWPEDLHTDL
EQLEQLVNGDINTYTLEKRYYTRNGEVVWALLAVSVVRHADGSPLYFIAQIEDINDLKQT
EWVNKRLMERITLANEAGGIGIWEWDLEPDIISWDKRMFELYEIPPHIKPTWQLWHDTML
PEDRAHAEQVLRDSLISRLPFKLEFRIRVKEGVRHIRSLANRVLNKQGEVERLLGINMDM
TEVKQLNEALFQEKERLHITLDSIGEAVLCTDINMNVTFMNPVAEKMSGWLQTEAIGQPI
LKVLHITFGEKGPLMENIHSGDMSRSDIDQDVVLNCRTGGNFDIHYSITPLSTLDGQNIG
SVIVIQDVTESRKMLRQLSYSASHDALTHLANRVSFENHLKRLLQTVRETRQRHALVFID
LDRFKAVNDTAGHAAGDALLRELSSLMLTMLRSSDVLARLGGDEFGLLLPDCNIESARYI
AGRLIHTINDYHFMWEGRLHRIGASAGITLIDENNHQASEVMSQADIACYASKNSGRGMV
TVYEPQQERDHNTRSMMSLDEQWHMIKDNHLMMIARSVASPRIPESSNFWLLSLRLWTSQ
GEVQEEHAFRSGLAEPELLHALDRRIFNEFFRASAAQVASKGMGVALPLSEAGLASVTLV
DELLDLLDKSPMPGRLLHLVISADVVCSPDKNLQRSLQKLRQAGCRVIFSQVGRDMNVFT
HLTSNMADYLMLDAEVVSNVYGNLMDEMMVTIVQGHAQRLGMKTIAGPCNQPIMMDTLSG
IGIDFIFGDTIAEPQPLDLLLNTSYFAIN