Protein Info for EX28DRAFT_0468 in Enterobacter asburiae PDN3

Annotation: hydroxyethylthiazole kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00694: hydroxyethylthiazole kinase
  • amino acids 11 to 255 (245 residues), 299.4 bits, E=1e-93
• PF02110: HK
  • amino acids 11 to 254 (244 residues), 325.4 bits, E=1.1e-101

Best Hits

Swiss-Prot: 86% identical to THIM_ENT38: Hydroxyethylthiazole kinase (thiM) from Enterobacter sp. (strain 638)

KEGG orthology group: K00878, hydroxyethylthiazole kinase [EC: 2.7.1.50] (inferred from 93% identity to enc:ECL_03416)

MetaCyc: 70% identical to hydroxyethylthiazole kinase (Escherichia coli K-12 substr. MG1655)
Hydroxyethylthiazole kinase. [EC: 2.7.1.50]; 2.7.1.50 [EC: 2.7.1.50]; Choline kinase. [EC: 2.7.1.50, 2.7.1.32]

Predicted SEED Role

"Hydroxyethylthiazole kinase (EC 2.7.1.50)" in subsystem Thiamin biosynthesis (EC 2.7.1.50)

MetaCyc Pathways

• thiamine diphosphate salvage II (5/5 steps found)
• prenylated FMNH₂ biosynthesis (3/3 steps found)
• thiamine diphosphate salvage V (3/3 steps found)
• thiamine diphosphate salvage IV (yeast) (4/7 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (4/8 steps found)
• superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)
• plasmalogen biosynthesis I (aerobic) (1/16 steps found)

KEGG Metabolic Maps

• Glycerophospholipid metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.50

Use Curated BLAST to search for 2.7.1.32 or 2.7.1.50

Protein Sequence (256 amino acids)

>EX28DRAFT_0468 hydroxyethylthiazole kinase (Enterobacter asburiae PDN3)
MQPDLLDLHVLHHFRTRSPLTHCMTNDVVQTFTANVLLALGASPAMVIEAEEAEQFAGIA
DALLINVGTLTSPRAQAMRRAIESAVAADKPWTLDPVAAGALTFRTRFCHQILALKPAAI
RGNASEILALAGMSAGGRGVDTTDTAASAVPAARALARQTNAVVIVTGEVDYITDGQRTR
TVTGGDPLMTRVVGTGCALSAVVAASCSLPGDRMDNVAAACGWMKRAGTVAVAQSRGPGS
FASAFLDALYTLEEQA