Protein Info for EX28DRAFT_0451 in Enterobacter asburiae PDN3

Annotation: sucrose-6-phosphate hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01322: sucrose-6-phosphate hydrolase
  • amino acids 21 to 450 (430 residues), 451.9 bits, E=1.1e-139
• PF00251: Glyco_hydro_32N
  • amino acids 28 to 325 (298 residues), 350.6 bits, E=9.9e-109
• PF08244: Glyco_hydro_32C
  • amino acids 351 to 474 (124 residues), 75.7 bits, E=4.1e-25

Best Hits

KEGG orthology group: K01193, beta-fructofuranosidase [EC: 3.2.1.26] (inferred from 83% identity to kpn:KPN_03261)

Predicted SEED Role

"Sucrose-6-phosphate hydrolase (EC 3.2.1.B3)" (EC 3.2.1.B3)

MetaCyc Pathways

• sucrose degradation III (sucrose invertase) (4/4 steps found)
• sucrose degradation V (sucrose α-glucosidase) (3/5 steps found)
• inulin degradation (1/3 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.26, 3.2.1.B3

Use Curated BLAST to search for 3.2.1.26 or 3.2.1.B3

Protein Sequence (477 amino acids)

>EX28DRAFT_0451 sucrose-6-phosphate hydrolase (Enterobacter asburiae PDN3)
MMYSIAKAEQELQARQGSLNLRWYPRYHLAARAGWMNDPNGLVWFDGWYHAFYQHHPYST
QWGPMHWGHARSKDLVRWEHLPVALAPEGPEDKDGCFSGSAVVDGDTLALIYTGHKFHGD
PDDEANLYQVQCLATSRDGVHFTRLGTIVDTPPGLHHFRDPKVWREGEDWYMVVGARDGE
TGQVRLYRSADLREWLDMGVLAVAEKEMGYMWECPDFFTLNGKRVLMFSPQGLAAEGFKN
RNLFQSGYLLGEWQPGQPFVREGEFVEMDRGHDFYAPQSFLTPDGRRIVIGWLDMWESPL
PEQQDGWAGMLSLPRELTLSEDNRLQMRAAREVESLRGAWFPWPISTLNNQQIVQVESCD
ALEVILRWDCANSSAEQYGICLGDGLRVYVDAQMQHLVLERHYPQYGLCGTRSVALNLND
TLNLRLFFDGSSVEVFVNDGDACLSSRIYPEAGARELALFAWTGSASLIDAGAWQLE