Protein Info for EX28DRAFT_0387 in Enterobacter asburiae PDN3

Annotation: Membrane-associated phospholipid phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 241 transmembrane" amino acids 9 to 28 (20 residues), see Phobius details amino acids 66 to 84 (19 residues), see Phobius details amino acids 96 to 118 (23 residues), see Phobius details amino acids 151 to 169 (19 residues), see Phobius details amino acids 174 to 193 (20 residues), see Phobius details amino acids 199 to 219 (21 residues), see Phobius details PF01569: PAP2" amino acids 143 to 211 (69 residues), 32.4 bits, see alignment E=3.6e-12

Best Hits

Swiss-Prot: 74% identical to LPXT_ECOLI: Lipid A 1-diphosphate synthase (lpxT) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 92% identity to enc:ECL_03493)

MetaCyc: 74% identical to lipid A-core phosphotransferase (Escherichia coli K-12 substr. MG1655)
Undecaprenyl-diphosphatase. [EC: 3.6.1.27]; RXN-16281 [EC: 3.6.1.27, 2.7.4.29]; 2.7.4.29 [EC: 3.6.1.27, 2.7.4.29]

Predicted SEED Role

"Putative membrane protein"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.27

Use Curated BLAST to search for 2.7.4.29 or 3.6.1.27

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (241 amino acids)

>EX28DRAFT_0387 Membrane-associated phospholipid phosphatase (Enterobacter asburiae PDN3)
MQNVMTTRFPLILLLNVAGLALFFSWYIPADHGFWFPLDSAIFHFFNQELVKSNAFLWLV
AITNNRAFDGFSLLAMGCLMLSFWLKEDKAGRRRILIIGLVMLLFAVVINQLATAIIPVK
RSSPTLFFTDIYRVSELLHIPTKDASKDSFPGDHGMMLLIFSAIMLRYFGRKAFAIALII
FVVFAFPRVMIGAHWFTDIAVGSLSVALIALPWCLMTPLSDRIIALFDRYLPGRAQQQLN
K