Protein Info for EX28DRAFT_0368 in Enterobacter asburiae PDN3

Annotation: malate:quinone-oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06039: Mqo
  • amino acids 31 to 519 (489 residues), 794.9 bits, E=2.4e-243
• TIGR01320: malate dehydrogenase (acceptor)
  • amino acids 32 to 517 (486 residues), 706.1 bits, E=1.1e-216
• PF01266: DAO
  • amino acids 33 to 305 (273 residues), 34.6 bits, E=1.6e-12

Best Hits

Swiss-Prot: 93% identical to MQO_ENT38: Probable malate:quinone oxidoreductase (mqo) from Enterobacter sp. (strain 638)

KEGG orthology group: K00116, malate dehydrogenase (quinone) [EC: 1.1.5.4] (inferred from 98% identity to enc:ECL_03512)

MetaCyc: 84% identical to malate:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
MALATE-DEHYDROGENASE-ACCEPTOR-RXN [EC: 1.1.5.4]

Predicted SEED Role

"Malate:quinone oxidoreductase (EC 1.1.5.4)" in subsystem TCA Cycle (EC 1.1.5.4)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle VI (Helicobacter) (8/9 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.4

Protein Sequence (549 amino acids)

>EX28DRAFT_0368 malate:quinone-oxidoreductase (Enterobacter asburiae PDN3)
MKKMTAMLFSLAVGLNTVSMAAKADAPKEQETDVLLIGGGIMSATLGTYLQELQPDWSMT
MVERLDGVAQESSNGWNNAGTGHSALMELNYTPQKKDGSISIEKAVEINEAFQVSRQFWS
HQVNSGVMHDPHSFINTVPHMSFVWGEQNVNFLRARYAALQQSTLFRGMKYSEDHAQIKE
WAPLVMEGRDPNQKVAATRTEIGTDVNYGEITRQLVTSLKKKENFNLQLSTEVRGFKRNA
DNSWSVTVADLKNNEAEHVIKAKFVFIGAGGAALKLLQESGIPEADDYAGFPVGGQFLVS
DNPEVVNRHLAKVYGQASVGAPPMSVPHIDTRMLDGKRVVLFGPFATFSTKFLKNGSLWD
LLSATTTSNVKPMMDVGLDNFDLVKYLISQVMLSDDDRFEALKEYYPQAKKEDWRLWQAG
QRVQIIKRDPKEGGVLRLGTEVVSDKDGTIAALLGASPGASTAAPIMLHLMEKVFKDKVA
SPEWQAKLKTIIPSYGTKLNGNVSATEQELEYTSRVLQLQYIKPQAADAAPKAELKPQPE
NKPVADIAL