Protein Info for EX28DRAFT_0357 in Enterobacter asburiae PDN3

Annotation: Signal transduction histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 948 signal peptide" amino acids 1 to 41 (41 residues), see Phobius details transmembrane" amino acids 314 to 334 (21 residues), see Phobius details PF00512: HisKA" amino acids 470 to 534 (65 residues), 64.2 bits, see alignment 1.9e-21 PF02518: HATPase_c" amino acids 581 to 691 (111 residues), 104.8 bits, see alignment E=7.3e-34 PF09456: RcsC" amino acids 709 to 799 (91 residues), 120.2 bits, see alignment E=5.7e-39 PF00072: Response_reg" amino acids 827 to 936 (110 residues), 101.9 bits, see alignment E=4.6e-33

Best Hits

Swiss-Prot: 86% identical to RCSC_ECOLI: Sensor histidine kinase RcsC (rcsC) from Escherichia coli (strain K12)

KEGG orthology group: K07677, two-component system, NarL family, capsular synthesis sensor histidine kinase RcsC [EC: 2.7.13.3] (inferred from 96% identity to enc:ECL_03522)

MetaCyc: 86% identical to histidine kinase RcsC (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Two-component sensor protein RcsC (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (948 amino acids)

>EX28DRAFT_0357 Signal transduction histidine kinase (Enterobacter asburiae PDN3)
MKYLVSFRTTLKVSRYLFRALALLLWLLVALFSVFYIVNALHQKEAEIRQEFNLSSDQAQ
RYIQRTSDVMKELKYIAENRLTAENGILALRGRNDNTEVPDFQPLFPDSDCSTMSNTWRG
SLESLAWFMRYWRDNFSAAYDLNRVFLIGSENLCMADFGLRDVPVERDDALKSLHERIVK
YRNAPQDERGNNIFWVSQGPRPGVGYFYALTPVYLGNRLQALLGIEQTIRMENFFTPGSL
PMGVTILDENGHQLISLAGPDNRLNVEPHWMQERAWFGYTSGFRELVLKKSLPPSSLSIV
YSLPVDMVLERIRILIMNAILLNLIAGGALFTLARMYERKIFIPAESDAQRLEEHEQFNR
KIVASAPVGICILRTQDGTNILSNELAHNYLNMLTHEDRQRLTQIICGQQVNFVDVLTST
HTNLQISFVHSRYRNENVAICVLVDVSARVKMEESLQEMAQAAEQASQSKSMFLATVSHE
LRTPLYGIIGNLDLLQTKELPKGVDRLVTAMNNSSSLLLKIISDILDFSKIESEQLKIEP
REFSPREVMNHISANYLPLVVRKQLGLYCFIEPDVPLTLHGDPMRLQQVISNLLSNAIKF
TDVGCIVLHVCRAGDYLSIRVRDTGVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAI
CEKLVSMMDGDISVDTEPGMGSQFTIRIPLYSAQYPAKATVDGLSDKRCWLAVHNASLND
YLTALLAHSGVRVCRYEGQTPDVDDVLIADEMPEQPWQGRGSVLFCRRHIGIPVERAPGE
WVHSVATPHELLSLLARIYKVELEERDGAGGLPSPESLASVNDDMMILVVDDHPINRRLL
ADQLGSLGYQCKTANDGVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVV
GVTANALAEEKQRCLESGMDSCLSKPVTLDVLKQTLSIYADRVRKTRI