Protein Info for EX28DRAFT_0354 in Enterobacter asburiae PDN3

Annotation: ribonucleoside-diphosphate reductase, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03477: ATP-cone
  • amino acids 6 to 92 (87 residues), 50.5 bits, E=3.6e-17
• PF00317: Ribonuc_red_lgN
  • amino acids 141 to 219 (79 residues), 56.7 bits, E=3.2e-19
• TIGR02506: ribonucleoside-diphosphate reductase, alpha subunit
  • amino acids 145 to 735 (591 residues), 598.5 bits, E=7.6e-184
• PF02867: Ribonuc_red_lgC
  • amino acids 223 to 732 (510 residues), 473.9 bits, E=9.8e-146

Best Hits

Swiss-Prot: 95% identical to RIR1_SALTY: Ribonucleoside-diphosphate reductase 1 subunit alpha (nrdA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 95% identity to esc:Entcl_1485)

MetaCyc: 95% identical to ribonucleoside-diphosphate reductase 1 subunit alpha (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Ribonucleotide reductase of class Ia (aerobic), alpha subunit (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (8/8 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (7/7 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)

KEGG Metabolic Maps

• Glutathione metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.4.1

Use Curated BLAST to search for 1.17.4.1

Protein Sequence (761 amino acids)

>EX28DRAFT_0354 ribonucleoside-diphosphate reductase, alpha subunit (Enterobacter asburiae PDN3)
MNQSLLVTKRDGTTERINLDKIHRVLDWAAEGLNNVSISQVELRSHIQFYDGIKTSDIHE
TIIKAAADLISREAPDYQYLAARLAIFHLRKKAYGQFEPPKLYDHVVKMVELGKYDTHLL
EDYTEEEFEQMNGFIDHWRDMNFSYAAVKQLEGKYLVQNRVTGEIYESAQFLYILVAACL
FSNYPRDTRLEYVKRFYDAVSTFKISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINAT
SSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHTGCIPFYKHFQTAVKSCSQGGVRGG
AATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMDYGVQINKLMYTRLLKGEDITLFSPSD
VPGLYDAFFADQDEFERLYTKYEKDDSIRKQRVKAVDLFSLMMQERASTGRIYIQNVDHC
NTHSPFDPVVAPVRQSNLCLEIALPTKPLEDVNDENGEIALCTLSAFNLGAIKSLDELEE
LAVLAVRALDALLDYQDYPIPAAKRGAMGRRTLGIGVINFAYWLAKNGKRYSDGSANNLT
HQTFEAIQYYLMKASNELAKEQGACPWFNETTYAKGILPIDTYKKDLDAIVSEPLHLDWE
GLRESIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRGHVSIKASKDGVLRQVVPDYE
TLGNNYELLWEMPNNDGYLQLVGIMQKFIDQSISANTNYDPTRFPSGKVPMQQLLKDLLT
AYKFGVKTLYYHNTRDGAEDAQDDMVPSIQDDGCESGACKI