Protein Info for EX28DRAFT_0286 in Enterobacter asburiae PDN3

Annotation: tRNA pseudouridine(38-40) synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 270 TIGR00071: tRNA pseudouridine(38-40) synthase" amino acids 11 to 247 (237 residues), 266.3 bits, see alignment E=1.2e-83 PF01416: PseudoU_synth_1" amino acids 17 to 112 (96 residues), 50.3 bits, see alignment E=1.5e-17 amino acids 151 to 253 (103 residues), 106.7 bits, see alignment E=4.4e-35

Best Hits

Swiss-Prot: 95% identical to TRUA_ENT38: tRNA pseudouridine synthase A (truA) from Enterobacter sp. (strain 638)

KEGG orthology group: K06173, tRNA pseudouridine synthase A [EC: 5.4.99.12] (inferred from 99% identity to enc:ECL_03667)

MetaCyc: 92% identical to tRNA pseudouridine38-40 synthase (Escherichia coli K-12 substr. MG1655)
tRNA-pseudouridine synthase I. [EC: 5.4.99.12]

Predicted SEED Role

"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (270 amino acids)

>EX28DRAFT_0286 tRNA pseudouridine(38-40) synthase (Enterobacter asburiae PDN3)
MSDVEQKPVHRIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALSQVANEPINVLCAGRTD
AGVHGTGQVVHFETTAVRKDAAWTLGVNANLPGDIAVRWVKAVPDDFHARFSATARRYRY
VIYNQRLRPAVLSQGVTHFYEPLDAERMHRAAQCLIGENDFTSFRAVQCQSRTPWRNVMH
INVSRFGAYVVVDIKANAFVHHMVRNIVGSLMEVGAGHQPESWIADLLAAKDRTLAAATA
KAEGLYLVSVDYPDRFDLPKPPMGPLFLAD