Protein Info for EX28DRAFT_0280 in Enterobacter asburiae PDN3

Annotation: tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 665 transmembrane" amino acids 267 to 284 (18 residues), see Phobius details PF05430: Methyltransf_30" amino acids 118 to 244 (127 residues), 157.4 bits, see alignment E=1.6e-50 PF01266: DAO" amino acids 267 to 631 (365 residues), 174.8 bits, see alignment E=3.9e-55 TIGR03197: tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain" amino acids 267 to 661 (395 residues), 502.8 bits, see alignment E=3.8e-155

Best Hits

Swiss-Prot: 79% identical to MNMC_ENT38: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC) from Enterobacter sp. (strain 638)

KEGG orthology group: None (inferred from 90% identity to enc:ECL_03673)

MetaCyc: 76% identical to fused tRNA 5-aminomethyl-2-thiouridylate methyltransferase and tRNA 5-aminomethyl-2-thiouridylate synthase (Escherichia coli K-12 substr. MG1655)
tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase. [EC: 2.1.1.61]; RXN0-7081 [EC: 2.1.1.61]

Predicted SEED Role

"tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61)" (EC 2.1.1.61)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.61

Use Curated BLAST to search for 2.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (665 amino acids)

>EX28DRAFT_0280 tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (Enterobacter asburiae PDN3)
MKQNAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLDGNQLSTRFPEHPRSLF
VVAESGFGTGLNFLTLWQAFDQFHAAHPEATLQRLHFISFEKFPLTAHDLRLAHQRWPEL
AAWAEQLQAQWPPHIGGCHRLILDGGRVTLDLWLGDINDLTDTLDDSMNQTVDAWFLDGF
APAKNPDMWSQHLFSAMARLARPGATLATFTSAGFVRRGLQEAGFTMQKTKGFGRKRDML
VGRMEQTLDVPARAPWFARRASASREVGIVGGGIASALLSLALLHRGWQVTLYCADDAPA
SGASGNRQGALYPLLSAHDPALFRFFPAAFTFARRLYDALPVAFDHDWCGVTQLGWDEKS
QQKITQMLSLGLPDEIARAVSAQEVADTAGVETGCGGIQYPLGGWLCPAELTAAAIALAQ
SRGLTAHYAHKVESLSRTERWNLRFADGKEASHASVVLANGHNISQFTQTETLPVYPVGG
QVSHIPSASELSKLRQVLCYDGYLTPQNPSNGHHCIGASYHRGEADMRYSDADQAQNRQR
LIDCFPDAAWAKEVDVSEGNARCGVRCATRDHLPMAGNVPDYDATLEAYQDLADNRDNAV
SAPVYPELYMLGGLGSRGLCSAPLLAEVLAAQMSDEPIPLDRVTLAGLNPNRLWVRKLLK
GKKVK