Protein Info for EX28DRAFT_0231 in Enterobacter asburiae PDN3

Annotation: nucleoside transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 395 transmembrane" amino acids 6 to 23 (18 residues), see Phobius details amino acids 30 to 50 (21 residues), see Phobius details amino acids 87 to 108 (22 residues), see Phobius details amino acids 166 to 187 (22 residues), see Phobius details amino acids 193 to 213 (21 residues), see Phobius details amino acids 242 to 264 (23 residues), see Phobius details amino acids 270 to 292 (23 residues), see Phobius details amino acids 336 to 358 (23 residues), see Phobius details amino acids 374 to 394 (21 residues), see Phobius details TIGR00804: nucleoside transporter, NupC family" amino acids 5 to 393 (389 residues), 467 bits, see alignment E=2.9e-144 PF01773: Nucleos_tra2_N" amino acids 10 to 82 (73 residues), 74.4 bits, see alignment E=1.2e-24 PF07670: Gate" amino acids 91 to 191 (101 residues), 58.6 bits, see alignment E=1.2e-19 PF07662: Nucleos_tra2_C" amino acids 193 to 391 (199 residues), 204 bits, see alignment E=3.5e-64

Best Hits

Swiss-Prot: 94% identical to NUPC_ECOLI: Nucleoside permease NupC (nupC) from Escherichia coli (strain K12)

KEGG orthology group: K11535, nucleoside transport protein (inferred from 98% identity to ent:Ent638_2927)

MetaCyc: 94% identical to nucleoside:H+ symporter NupC (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-108A; TRANS-RXN-108B; TRANS-RXN-108C; TRANS-RXN-108D; TRANS-RXN-108E; TRANS-RXN-108F; TRANS-RXN-108G; TRANS-RXN-108H; TRANS-RXN-108I; TRANS-RXN-476

Predicted SEED Role

"Nucleoside permease NupC" in subsystem Deoxyribose and Deoxynucleoside Catabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (395 amino acids)

>EX28DRAFT_0231 nucleoside transporter (Enterobacter asburiae PDN3)
MDRVLHFVLALVVVTALALLVSTDRKKIRIRYVVQLLVIEVLLAWFFLNSNVGLGFVKGF
SEMFEKLLGFANEGTNFVFGKMNDEGLAFFFLKVLCPIVFISALIGILQHIRVLPFVIRG
IGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGKMSRNRMYTMAATAMSTVSMS
IVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMSNLHEGQSFFEMLG
EYILAGFKVAIIVAAMLIGFIALISALNALFAAVLGISFQGILGYIFYPVAWVMGVPAHE
ALQVGSIMATKLVSNEFVAMMDLQKIASTLSPRAEGILSVFLVSFANFSSIGIIAGAIKG
LNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL