Protein Info for EX28DRAFT_0210 in Enterobacter asburiae PDN3

Annotation: pyridoxal kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00687: pyridoxal kinase
  • amino acids 15 to 274 (260 residues), 165.2 bits, E=1.1e-52
• PF08543: Phos_pyr_kin
  • amino acids 87 to 264 (178 residues), 82.5 bits, E=3.2e-27
• PF00294: PfkB
  • amino acids 135 to 255 (121 residues), 42.5 bits, E=5.5e-15

Best Hits

Swiss-Prot: 71% identical to PDXK_SALTY: Pyridoxine/pyridoxal/pyridoxamine kinase (pdxK) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00868, pyridoxine kinase [EC: 2.7.1.35] (inferred from 94% identity to enc:ECL_03748)

MetaCyc: 67% identical to pyridoxine/pyridoxal/pyridoxamine kinase (Escherichia coli K-12 substr. MG1655)
Hydroxymethylpyrimidine kinase. [EC: 2.7.1.49]; Pyridoxal kinase. [EC: 2.7.1.49, 2.7.1.35]; 2.7.1.35 [EC: 2.7.1.49, 2.7.1.35]; 2.7.1.35 [EC: 2.7.1.49, 2.7.1.35]; 2.7.1.35 [EC: 2.7.1.49, 2.7.1.35]

Predicted SEED Role

"Pyridoxal kinase (EC 2.7.1.35)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 2.7.1.35)

MetaCyc Pathways

• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (12/12 steps found)
• pyridoxal 5'-phosphate salvage I (6/6 steps found)
• pyridoxal 5'-phosphate salvage II (plants) (8/9 steps found)
• thiamine diphosphate salvage II (5/5 steps found)
• hydroxymethylpyrimidine salvage (2/2 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (5/8 steps found)
• thiamine diphosphate salvage IV (yeast) (4/7 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (4/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism
• Vitamin B6 metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.35, 2.7.1.49

Use Curated BLAST to search for 2.7.1.35 or 2.7.1.49

Protein Sequence (278 amino acids)

>EX28DRAFT_0210 pyridoxal kinase (Enterobacter asburiae PDN3)
MEMILFRDNTRAQQTDIVAVQSQVVYGSVGNSIAVPNIRTHRLNVTAVPTVLFSNTPHYD
TFYGGVIPDEWFSGYLKALEEREILRELKAVTTGYMGSASQIVLLAQWLKAVKAQHPDLL
VLVDPVIGDIDSGMYVKPEIPQAYREHLLPLAQGITPNVYELEVLSGKPCRTPESAIAAA
QGLLSDSLKWVAITSAPVADDPENIHVVLVTNEGVTVSAHPRVETDLKGTGDLFCSELVS
GIVEGKTVADAIRMAGDRVTDVMLYTQSKGYDELILPA