Protein Info for EX28DRAFT_0191 in Enterobacter asburiae PDN3

Annotation: The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1037 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 340 to 359 (20 residues), see Phobius details amino acids 366 to 387 (22 residues), see Phobius details amino acids 393 to 413 (21 residues), see Phobius details amino acids 438 to 459 (22 residues), see Phobius details amino acids 471 to 496 (26 residues), see Phobius details amino acids 541 to 559 (19 residues), see Phobius details amino acids 872 to 890 (19 residues), see Phobius details amino acids 897 to 917 (21 residues), see Phobius details amino acids 923 to 947 (25 residues), see Phobius details amino acids 969 to 988 (20 residues), see Phobius details amino acids 1004 to 1026 (23 residues), see Phobius details PF00873: ACR_tran" amino acids 1 to 1027 (1027 residues), 1419 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1035 (1035 residues), 1790.2 bits, see alignment E=0 PF03176: MMPL" amino acids 332 to 514 (183 residues), 31.8 bits, see alignment E=1.1e-11

Best Hits

Swiss-Prot: 91% identical to ACRD_ECOLI: Probable aminoglycoside efflux pump (acrD) from Escherichia coli (strain K12)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 66% identity to asa:ASA_1419)

MetaCyc: 66% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1037 amino acids)

>EX28DRAFT_0191 The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family (Enterobacter asburiae PDN3)
MANFFIDRPIFAWVLAILLCLTGVLAITSLPVEQYPDLAPPNVRVTANYPGASAQTLENT
VTQVIEQNMTGLDNLMYMSSQSSATGQATVTLSFKAGTDPDEAVQQVQNQLQSAMRKLPQ
AVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVASNIQDPISRINGVGDIDAYGS
QYSMRIWLDPNKLNSVQMTATDVTNAIKAQNAQIAVGQLGGTPSVDKQALNATINSQSLL
QTPDQFRNITLRVNQDGSEVRLGDVATVEMGAEKYDYLSRFNGNQASGLGIKLASGANEM
ATAKLVLDRLDELSQYFPHGLEYKVAYETTSFVKASIEDVVKTLLEAIALVFLVMYLFLQ
NFRATLIPTIAVPVVLLGTFAVLYAFGYSINTLTMFAMVLAIGLLVDDAIVVVENVERIM
SEEGLSPRQATRKSMGQIQGALVGIAMVLSAVFIPMAFFGGTTGAIYRQFSITIVSAMVL
SVLVALILTPALCATILKPLHKGEQHGQKGFFGWFNRMFNRNAARYESAVGGVLHRSLRW
MMIYVVLLGGMVWLFMHLPTSFLPMEDRGMFTTSVQLPSGATQQQTLKVVQKVEQYFFTK
EKDNVVSVFSTVGSGPGGNGQNVARMFVRLKDWDERDTKTGTSFAIIERATKAFAHIKEA
RVFASSPPAISGLGSSAGFDMELQDHAGAGHTALMAARDKLLALAGNDPGLTRVRHNGLD
DSPQLQVDIDQRKAQALGVSINDINDTLQTAWGSSYVNDFMDRGRVKKVYVQSAAPFRML
PDDINRWFVRNNAGGMVPFSAFATSHWESGSPRLERYNGYSAVEIVGEAAPGVSTGTAMD
TMEKLVQQLPAGFGLEWTAMSYQERLSGAQAPALYALSLLVVFLCLAALYESWSVPFSVM
LVVPLGVIGALLATWMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNAKGHELMAA
TLHACRQRLRPILMTSLAFVFGVLPMATSTGAGSSSQHAVGTGVMGGMISATVLAIYFVP
LFFVLVRRRFPLKERAE