Protein Info for EX28DRAFT_0175 in Enterobacter asburiae PDN3

Annotation: phosphotransferase system, cellobiose specific, IIC component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 439 transmembrane" amino acids 31 to 51 (21 residues), see Phobius details amino acids 71 to 93 (23 residues), see Phobius details amino acids 104 to 121 (18 residues), see Phobius details amino acids 141 to 159 (19 residues), see Phobius details amino acids 180 to 200 (21 residues), see Phobius details amino acids 226 to 246 (21 residues), see Phobius details amino acids 287 to 307 (21 residues), see Phobius details amino acids 332 to 355 (24 residues), see Phobius details amino acids 364 to 388 (25 residues), see Phobius details amino acids 394 to 412 (19 residues), see Phobius details TIGR00359: PTS system, cellobiose-specific IIC component" amino acids 12 to 424 (413 residues), 439.3 bits, see alignment E=1.6e-135 TIGR00410: PTS system, lactose/cellobiose family IIC component" amino acids 12 to 424 (413 residues), 439.3 bits, see alignment E=1.6e-135 PF02378: PTS_EIIC" amino acids 33 to 351 (319 residues), 202.5 bits, see alignment E=4.7e-64

Best Hits

Swiss-Prot: 40% identical to PTEC_BACSU: PTS system oligo-beta-mannoside-specific EIIC component (gmuC) from Bacillus subtilis (strain 168)

KEGG orthology group: K02761, PTS system, cellobiose-specific IIC component (inferred from 97% identity to enc:ECL_03783)

Predicted SEED Role

"PTS system, cellobiose-specific IIC component (EC 2.7.1.69)" in subsystem Beta-Glucoside Metabolism (EC 2.7.1.69)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (439 amino acids)

>EX28DRAFT_0175 phosphotransferase system, cellobiose specific, IIC component (Enterobacter asburiae PDN3)
MNNVLGFLEAKLMPLAAKTAQQRHLGAIRGAYVSFMPFIIVGSILLVISSFPNQAYQQFM
SQAFGESWSAIIEIPFNAVFSTMSLFISFLVAFRLAEHYGEDRISCGILALVAFLILTPF
IKVAENGGITVIPVEWIGSKGLFVAMIGSLLWTELFCWLKRKKLVIKMPDGVPPAVQESF
AALIPALLVMILVLGIRIVFENTHYNTIHQFIYEVVATPVRHYGTSYFGALMTVFSITIL
WSVGINSGSMINGIIRPLWMENQTDNIAAIQAGTTPPHIITEQFFDMIWMGGAGATLSLV
IAMLIFARSKNMREVARLGAGASVFNINEPILFGLPVIMNPIMLIPFNLVPLVLVTVQYA
AMKIGAVAVTTGVFIPWTLPPVISGFIVTGHLSGSVMQLINLLIGAMLYLPFMRIVDKQY
RAAEMATVTRTDTTLAKQE