Protein Info for EX28DRAFT_0164 in Enterobacter asburiae PDN3

Annotation: FOG: EAL domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 746 transmembrane" amino acids 14 to 34 (21 residues), see Phobius details amino acids 45 to 74 (30 residues), see Phobius details amino acids 85 to 105 (21 residues), see Phobius details amino acids 126 to 147 (22 residues), see Phobius details amino acids 168 to 190 (23 residues), see Phobius details amino acids 211 to 232 (22 residues), see Phobius details amino acids 238 to 258 (21 residues), see Phobius details amino acids 265 to 282 (18 residues), see Phobius details amino acids 294 to 317 (24 residues), see Phobius details PF05231: MASE1" amino acids 15 to 320 (306 residues), 127.6 bits, see alignment E=5.5e-41 PF00563: EAL" amino acids 499 to 730 (232 residues), 213.6 bits, see alignment E=2.8e-67

Best Hits

Swiss-Prot: 62% identical to PDEF_ECOLI: Cyclic di-GMP phosphodiesterase PdeF (pdeF) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 89% identity to enc:ECL_03795)

MetaCyc: 62% identical to cyclic di-GMP phosphodiesterase PdeF (Escherichia coli K-12 substr. MG1655)
Cyclic-guanylate-specific phosphodiesterase. [EC: 3.1.4.52]

Predicted SEED Role

"Putative cytochrome C-type biogenesis protein" in subsystem Biogenesis of c-type cytochromes

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.52

Use Curated BLAST to search for 3.1.4.52

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (746 amino acids)

>EX28DRAFT_0164 FOG: EAL domain (Enterobacter asburiae PDN3)
MNILAFLKKNENRWWALPLILPVVLLPVLSVANTFTQLGDGIAALYYLPLSFLLSLMMFF
GLEALPGIVLSLFIRYYPSVGMFETVAGILHFVVPLVLSWGGYRVFAPRRNMTAYGDVRL
MAQRIFWQVFCPATLFLVLFQFAVYLGVYESRQSLAGLNPLNIRTLINYQTLLVSGLTGV
PLSYLLIRLLRHPRYIKSLLSQIRSEIDKKVTVVELLVWAIALGGLLSLLLLPMNENSSI
FTTNYTLSLLMPVMLWGAMRFGYKLISLIWTPILLVSIHYFYRYIPVNPGYDIQLAITSS
SYLVFSFVVIYMSMLATRQRAANKRSRRLALLDPVVHMPNLRALSRDLAKNPWSALCLLR
IPELEILGRNYGVLLRIQYKQQLAQWVNGILQPNELVYHLTGYDLAVRLNAESHQQRIEA
LDEHIKQFRFVWDGMPLQPQVGVSYCYVRSPVNHLYLVLGELGVVADLSLSSNHPENLQQ
RGAVHLQRNLKDKVAMMSRLQKALDNDEFTLLVQPVRGLRGDCYHEVLLRMPDVNGLLIS
PDQFLPVAQEFGLSSRVDLWVLEHTLRFLAAHRERLPGQRFAINLAPSTVCRVQFPLEVS
RLLAKYAIEPWQLIFEVTECSTFCNAEQALHILRQLQKMGVRIAIDDFGTGYASYARLKS
VDADILKIDGSFIRNIVNNSLDYQIVASICHLARMKKMLVVAEYVESEEIRSAVHALGID
YVQGYLIGRPAPLESLLEAEAPTADA