Protein Info for EX28DRAFT_0147 in Enterobacter asburiae PDN3

Annotation: methylmalonic acid semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01722: methylmalonate-semialdehyde dehydrogenase (acylating)
  • amino acids 4 to 484 (481 residues), 675.3 bits, E=2.4e-207
• PF00171: Aldedh
  • amino acids 18 to 480 (463 residues), 465 bits, E=1.2e-143

Best Hits

Swiss-Prot: 60% identical to BAUC_PSEAE: Putative 3-oxopropanoate dehydrogenase (bauC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 97% identity to enc:ECL_03809)

MetaCyc: 54% identical to malonate-semialdehyde dehydrogenase (acetylating) (Halomonas sp. HTNK1)
Malonate-semialdehyde dehydrogenase (acetylating). [EC: 1.2.1.18]

Predicted SEED Role

"Methylmalonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.27)" in subsystem Inositol catabolism (EC 1.2.1.27)

MetaCyc Pathways

• myo-inositol degradation I (7/7 steps found)
• myo-, chiro- and scyllo-inositol degradation (7/10 steps found)
• β-alanine degradation I (1/2 steps found)
• β-alanine degradation II (1/2 steps found)
• L-valine degradation I (4/8 steps found)
• acrylate degradation I (1/5 steps found)
• propanoyl-CoA degradation II (1/5 steps found)
• 2,4-dinitrotoluene degradation (1/7 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Inositol phosphate metabolism
• Propanoate metabolism
• Valine, leucine and isoleucine degradation
• beta-Alanine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.18 or 1.2.1.27

Protein Sequence (501 amino acids)

>EX28DRAFT_0147 methylmalonic acid semialdehyde dehydrogenase (Enterobacter asburiae PDN3)
METVANFIHGECVTGSGQRVQAIFNPATGEQIRQVVMSTARETEQAIAAAQQALPAWARH
APLKRARVMFRFKALLEENMDRLARIISEEHGKVFSDAVGELTRGLEVVEFACGIPHLQK
GEHSANVGTGVDSHSLMQPLGVCAGITPFNFPAMVPMWMFPIALATGNTFVLKPSEKDPS
LALALAQLLKEAGLPDGVFNVVQGDKESVDVLLTDPRVQAVSFVGSTPIAEYIYQTASAH
GKRCQALGGAKNHCILMPDADMEMATNAIMGAAYGAAGERCMALSVVLAVGDKTADDLCA
RLEKQIAALRVGPGLDQSPENEMGPLISSAHRSKVLGYIDSGEAQGAKLRVDGRGFSVKG
HEQGYFVGPTLFDNVTPEMTIYKEEIFGPVLSVVRVADYSSAVDLINRHEYGNGSAIFTR
DGGCARRFCEEVLAGMVGVNVPIPVPMAFHSFGGWKRSIFGALNVHGSDGVRFYTRMKTI
TARWPEMQQETGAAFSMPTLG