Protein Info for EX28DRAFT_0133 in Enterobacter asburiae PDN3

Annotation: ribosome-associated GTPase EngA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03594: ribosome-associated GTPase EngA
  • amino acids 3 to 458 (456 residues), 594.5 bits, E=2.1e-182
• TIGR00231: small GTP-binding protein domain
  • amino acids 4 to 158 (155 residues), 76.4 bits, E=3.4e-25
  • amino acids 202 to 368 (167 residues), 77.3 bits, E=1.7e-25
• PF01926: MMR_HSR1
  • amino acids 5 to 119 (115 residues), 105.4 bits, E=9.8e-34
  • amino acids 205 to 322 (118 residues), 95.6 bits, E=1e-30
• PF02421: FeoB_N
  • amino acids 5 to 157 (153 residues), 58.6 bits, E=2.7e-19
  • amino acids 204 to 366 (163 residues), 47.4 bits, E=7.9e-16
• PF00009: GTP_EFTU
  • amino acids 204 to 370 (167 residues), 43.4 bits, E=1.5e-14
• PF14714: KH_dom-like
  • amino acids 378 to 458 (81 residues), 93.9 bits, E=2.9e-30

Best Hits

Swiss-Prot: 96% identical to DER_ENT38: GTPase Der (der) from Enterobacter sp. (strain 638)

KEGG orthology group: K03977, GTP-binding protein (inferred from 98% identity to enc:ECL_03853)

MetaCyc: 93% identical to 50S ribosomal subunit stability factor (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein EngA" in subsystem Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (490 amino acids)

>EX28DRAFT_0133 ribosome-associated GTPase EngA (Enterobacter asburiae PDN3)
MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGRAEVEGREFICIDTGGI
DGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADSAIAKHLRSREKPTFLVANKT
DGIDADQAVADFWSLGLGEIYPIAASHGRGVTSLLETVLLPWVDEVNPPEEVDEDAEYWA
QFEDGEEGEEEPEDDFNPQDLPIKLAIVGRPNVGKSTLTNRILGEERVVVYDMPGTTRDS
IYIPMQRDEREYVLIDTAGVRKRGKITDVVEKFSVIKTLQAIEDANVVLLVIDAREGISD
QDLSLLGFILNSGRSLVIVVNKWDGLSNEVREQVKETLDFRLGFIDFARVHFISALHGSG
VGNLFESVREAYDSSTRRQSTAMLTRIMNMAAEDHQPPLVRGRRVKLKYAHAGGYNPPIV
VIHGNQVKDLPDSYKRYLMNYFRKSLDVMGTPIRIQFKEGENPFANKRNTLTPNQMRKRK
RLIKHIKKSK