Protein Info for EX28DRAFT_0098 in Enterobacter asburiae PDN3

Annotation: Hemoglobin-like flavoprotein/Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 PF00042: Globin" amino acids 27 to 130 (104 residues), 63.4 bits, see alignment E=4.4e-21 PF00970: FAD_binding_6" amino acids 156 to 252 (97 residues), 37.3 bits, see alignment E=4.2e-13 PF00175: NAD_binding_1" amino acids 264 to 371 (108 residues), 66.5 bits, see alignment E=4.7e-22

Best Hits

Swiss-Prot: 89% identical to HMP_ECOL6: Flavohemoprotein (hmp) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K05916, nitric oxide dioxygenase [EC: 1.14.12.17] (inferred from 96% identity to enc:ECL_03889)

MetaCyc: 88% identical to nitric oxide dioxygenase (Escherichia coli K-12 substr. MG1655)
6,7-dihydropteridine reductase. [EC: 1.5.1.34]; Nitric oxide dioxygenase. [EC: 1.5.1.34, 1.14.12.17]

Predicted SEED Role

"Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17)" in subsystem Bacterial hemoglobins or Flavohaemoglobin or Glutaredoxins (EC 1.14.12.17)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.34

Use Curated BLAST to search for 1.14.12.17 or 1.5.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (396 amino acids)

>EX28DRAFT_0098 Hemoglobin-like flavoprotein/Flavodoxin reductases (ferredoxin-NADPH reductases) family 1 (Enterobacter asburiae PDN3)
MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFNMSNQRNGDQREALFNA
IAAYASNIENLAALLPAVEKIAQKHTSFQIQPEQYNIVGGHLLATLDEMFSPGQEVLDAW
GKAYGVLANVFINREAQIYSENASKNGGWEGTRAFRIVEKTPRSALITSFEFEPVDGQPV
ADYQPGQYLGVWLKPEGFPHQEIRQYSLTRKPNGKGYRIAVKREDGGQVSNWLHNEANVG
DVVHLAAPAGDFFMAVEANTPVTLISAGVGQTPMLAMLDTLAKANHGAQVNWFHAAENGD
VHAFADEVKTLGASLPRFTAHTWYRLPRDKDRAAARFDSEGLMNLSQMEGAFSAPDMQFY
VCGPVAFMQYAALQLVELGVNKDNIHYECFGPHKVL