Protein Info for EX28DRAFT_0080 in Enterobacter asburiae PDN3

Annotation: GTP-binding protein Era

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00231: small GTP-binding protein domain
  • amino acids 7 to 171 (165 residues), 136.2 bits, E=8.9e-44
• TIGR00436: GTP-binding protein Era
  • amino acids 9 to 280 (272 residues), 391.2 bits, E=2.4e-121
• PF00009: GTP_EFTU
  • amino acids 11 to 174 (164 residues), 30.6 bits, E=8.7e-11
• PF02421: FeoB_N
  • amino acids 11 to 165 (155 residues), 47.2 bits, E=6.1e-16
• PF01926: MMR_HSR1
  • amino acids 11 to 125 (115 residues), 89.3 bits, E=6.8e-29
• PF04548: AIG1
  • amino acids 11 to 113 (103 residues), 28.5 bits, E=3.4e-10
• PF07650: KH_2
  • amino acids 209 to 285 (77 residues), 71 bits, E=2e-23

Best Hits

Swiss-Prot: 95% identical to ERA_ENT38: GTPase Era (era) from Enterobacter sp. (strain 638)

KEGG orthology group: K03595, GTP-binding protein Era (inferred from 99% identity to enc:ECL_03904)

MetaCyc: 95% identical to 30S ribosomal subunit maturation GTPase Era (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein Era" in subsystem Bacterial Cell Division or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (301 amino acids)

>EX28DRAFT_0080 GTP-binding protein Era (Enterobacter asburiae PDN3)
MSEEKTYCGFIAIVGRPNVGKSTLLNNLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIY
VDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLRDGKTPVI
LAVNKVDNVQEKADLLPHLQWLGSQMNFLDIVPLSAETGLNVDTIAGIVRKHLPEAIHHF
PEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFQTNERGGYDINGLILVERE
GQKKMVIGNKGAKIKTIGIEARKDMMEMFEAPVHLELWVKVKSGWADDERALRSLGYGED
Q