Protein Info for EX28DRAFT_0076 in Enterobacter asburiae PDN3

Annotation: elongation factor 4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01393: elongation factor 4
  • amino acids 2 to 594 (593 residues), 998.9 bits, E=7e-305
• PF00009: GTP_EFTU
  • amino acids 2 to 181 (180 residues), 186.3 bits, E=1.1e-58
• TIGR00231: small GTP-binding protein domain
  • amino acids 5 to 172 (168 residues), 58.3 bits, E=8.2e-20
• PF03144: GTP_EFTU_D2
  • amino acids 205 to 275 (71 residues), 42.8 bits, E=1.4e-14
• PF00679: EFG_C
  • amino acids 399 to 481 (83 residues), 69.6 bits, E=4.8e-23
• PF06421: LepA_C
  • amino acids 487 to 592 (106 residues), 167 bits, E=3.1e-53

Best Hits

Swiss-Prot: 98% identical to LEPA_KLEP3: Elongation factor 4 (lepA) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 98% identity to enc:ECL_03907)

MetaCyc: 95% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (599 amino acids)

>EX28DRAFT_0076 elongation factor 4 (Enterobacter asburiae PDN3)
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVT
LDYKAADGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAM
EMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVRCSAKTGVGVTDVLERLVRDI
PAPEGDPDGPLQALIIDSWFDNYLGVVSLVRIKNGTMRKGDKIKVMSTGQVYNADRLGIF
TPKQVDRTELRCGEVGWLVCAIKDILGAPVGDTLTGARNPADKALPGFKKVKPQVYAGLF
PVSSDDYENFRDALGKLSLNDASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYD
LDLITTAPTVVYEVETTSKEVIYVDSPSKLPPLNNIQELREPIAECHMLLPQEFLGNVIT
LCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASNM
VRVDVLINGERVDALALITHNDNAPYRGRELVEKMKDLIPRQQFDIAIQAAIGNHIIARS
TVKQLRKNVLAKCYGGDVSRKKKLLQKQKEGKKRMKQVGNVELPQEAFLAILHVGKDGK