Protein Info for EX28DRAFT_0063 in Enterobacter asburiae PDN3

Annotation: Acyl-CoA synthetase (NDP forming)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 887 PF13380: CoA_binding_2" amino acids 13 to 130 (118 residues), 84.1 bits, see alignment E=2.5e-27 PF13607: Succ_CoA_lig" amino acids 147 to 280 (134 residues), 153.7 bits, see alignment E=6.3e-49 PF13549: ATP-grasp_5" amino acids 472 to 693 (222 residues), 246.8 bits, see alignment E=4.2e-77 PF13302: Acetyltransf_3" amino acids 725 to 863 (139 residues), 34.9 bits, see alignment E=6.1e-12 PF00583: Acetyltransf_1" amino acids 759 to 863 (105 residues), 41.4 bits, see alignment E=4.2e-14

Best Hits

Swiss-Prot: 86% identical to LYSAC_ECOLI: Peptidyl-lysine N-acetyltransferase PatZ (patZ) from Escherichia coli (strain K12)

KEGG orthology group: K09181, hypothetical protein (inferred from 97% identity to enc:ECL_03920)

MetaCyc: 86% identical to peptidyl-lysine N-acetyltransferase (Escherichia coli K-12 substr. MG1655)
2.3.1.-

Predicted SEED Role

"Protein acetyltransferase" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (887 amino acids)

>EX28DRAFT_0063 Acyl-CoA synthetase (NDP forming) (Enterobacter asburiae PDN3)
MSQRGLEALLRPKSIAVIGASMKPDRAGYLMMRNLLAGGFNGPVMPVTPSYKAVQGVLAW
PDVQSLPFVPDLAVLCTNAKRNLELLESLGKKGCKTCIILSSPPEQKAELLACASRYQMR
ILGPNSLGLLAPWQGLNASFSPVPIRKGKLAFISQSAAVSNTILDWAQQREMGFSYFIAL
GDSLDIDVDELLDFLARDGKTSAILLYLEHLSDARRFVSASRSASRNKPILVIKSGRSPA
AQRLLHSHSGMDPAWDAAIQRAGLLRVQDTHELFSAVETLSHMRPLRGEKLMIVSNGAAP
AALALDELWRRFGKLATLSEETLQRLKEALPTSVTPGNPLDLRDDASSDRYIRAISILLD
SQDFDALMIIHSPSAVAPGSESARALIDAVRNHPRGKYVTLLTNWCGEFSSQEARRLFSE
AGLPTYRTPEGTITAFMHMVEYRRNQKQLRETPALPGNLTANTVDVHRLLQQAIEEGATS
LDTHEVQPILGSYGMQTLPTWIASDSAEAVHIAEQIGYPVALKLRSPDIPHKSDVQGVML
YLRTATEVQQAADAIFDRVKMAWPQARIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIM
LGEGGVEWRPEEQAVVALPPLNMNLARYLIIQAIKSKKIRGRSALRPLDIAGLSQFLVQV
SNLIVDCAEIQRLDIHPLLASGNEFTALDVTLDIAPFEGDRESRLAIRPYPLHLEEWVEM
KNGERALFRPILPEDEPQLRAFISQVTKEDLYYRYFSEINEFTHDDLANMTQIDYDREMA
FVAVRRSDKGDEILGVTRAISDPDNVDAEFAVLVRSDLKGLGLGRRLLEKLISYTRDHGL
LRLNGITMPNNRGMVTLARKLGFDVDIQLDAGIVALSLSLTSADKRE