Protein Info for EFB2_04453 in Escherichia fergusonii Becca

Annotation: Lysine--tRNA ligase, heat inducible

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 505 TIGR00499: lysine--tRNA ligase" amino acids 15 to 504 (490 residues), 798 bits, see alignment E=1.6e-244 PF01336: tRNA_anti-codon" amino acids 68 to 146 (79 residues), 61.7 bits, see alignment E=7.6e-21 PF00152: tRNA-synt_2" amino acids 162 to 501 (340 residues), 323.9 bits, see alignment E=1.4e-100

Best Hits

Swiss-Prot: 100% identical to SYK2_ECOL6: Lysine--tRNA ligase, heat inducible (lysU) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K04567, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 100% identity to eco:b4129)

MetaCyc: 100% identical to lysyl-tRNA synthetase (Escherichia coli K-12 substr. MG1655)
Lysine--tRNA ligase. [EC: 6.1.1.6]; 2.7.7.- [EC: 6.1.1.6]; 2.7.7.- [EC: 6.1.1.6]; RXN-23921 [EC: 6.1.1.6]; RXN-23924 [EC: 6.1.1.6]; RXN-23927 [EC: 6.1.1.6]; RXN0-7397 [EC: 6.1.1.6]

Predicted SEED Role

"Lysyl-tRNA synthetase (class II) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.6

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (505 amino acids)

>EFB2_04453 Lysine--tRNA ligase, heat inducible (Escherichia fergusonii Becca)
MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDHTSDQLHEEFDAKDNQE
LESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARDSLPEGVYNDQFKKWDLGD
IIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGLQDQEVRYRQRYLDLIANDKS
RQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGASARPFITHHNALDLDMYLRIAP
ELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADYHDLIELTESLFRT
LAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIKKYRPETDMADLDNFDAAKALAESIGI
TVEKSWGLGRIVTEIFDEVAEAHLIQPTFITEYPAEVSPLARRNDVNPEITDRFEFFIGG
REIGNGFSELNDAEDQAERFQEQVNAKAAGDDEAMFYDEDYVTALEYGLPPTAGLGIGID
RMIMLFTNSHTIRDVILFPAMRPLK