Protein Info for EFB2_04222 in Escherichia fergusonii Becca

Annotation: Hemolysin, chromosomal

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1001 transmembrane" amino acids 281 to 303 (23 residues), see Phobius details amino acids 359 to 362 (4 residues), see Phobius details amino acids 367 to 387 (21 residues), see Phobius details PF02382: RTX" amino acids 269 to 577 (309 residues), 307.8 bits, see alignment E=1.8e-95 PF00353: HemolysinCabind" amino acids 594 to 620 (27 residues), 8.8 bits, see alignment (E = 0.00026) amino acids 701 to 735 (35 residues), 22.1 bits, see alignment (E = 1.8e-08) amino acids 710 to 744 (35 residues), 33.7 bits, see alignment (E = 4.3e-12) amino acids 728 to 762 (35 residues), 41.1 bits, see alignment (E = 2e-14) amino acids 746 to 780 (35 residues), 28 bits, see alignment (E = 2.6e-10) amino acids 790 to 819 (30 residues), 35.3 bits, see alignment (E = 1.4e-12) amino acids 802 to 830 (29 residues), 24.1 bits, see alignment (E = 4.4e-09) PF08339: RTX_C" amino acids 851 to 977 (127 residues), 47.3 bits, see alignment E=3.6e-16

Best Hits

Swiss-Prot: 97% identical to HLYAC_ECOLX: Hemolysin, chromosomal (hlyA) from Escherichia coli

KEGG orthology group: K11005, hemolysin A (inferred from 100% identity to eci:UTI89_C4926)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1001 amino acids)

>EFB2_04222 Hemolysin, chromosomal (Escherichia fergusonii Becca)
MHSAGQSTKDALKKAAEQTRNAGNRLILLIPKDYKGQGSSLNDLVRTADELGIEVQYDEK
NGTAITKQVFGTAEKLIGLTERGVTIFAPQLDKLLQKYQKAGNKLGGSAENIGDNLGKAG
SVLSTFQNFLGTALSSMKIDELIKKQKSGSNVSSSELAKASIELINQLVDTAASINNNVN
SFSQQLNKLGSVLSNTKHLNGVGNKLQNLPNLDNIGAGLDTVSGILSAISASFILSNADA
DTGTKAAAGVELTTKVLGNVGKGISQYIIAQRAAQGLSTSAAAAGLIASVVTLAISPLSF
LSIADKFKRANKIEEYSQRFKKLGYDGDSLLAAFHKETGAIDASLTTISTVLASVSSGIS
AAATTSLVGAPVSALVGAVTGIISGILEASKQAMFEHVASKMADVIAEWEKKHGKNYFEN
GYDARHAAFLEDNFKILSQYNKEYSVERSVLITQQHWDMLIGELASVTRNGDKTLSGKSY
IDYYEEGKRLERRPKEFQQQIFDPLKGNIDLSDSKSSTLLKFVTPLLTPGEEIRERRQSG
KYEYITELLVKGVDKWTVKGVQDKGSVYDYSNLIQHASVGNNQYREIRIESHLGDGDDKV
FLSAGSANIYAGKGHDVVYYDKTDTGYLTIDGTKATEAGNYTVTRVLGGDVKVLQEVVKE
QEVSVGKRTEKTQYRSYEFTHINGKNLTETDNLYSVEELIGTTRADKFFGSKFTDIFHGA
DGDDHIEGNDGNDRLYGDKGNDTLRGGNGDDQLYGGDGNDKLIGGTGNNYLNGGDGDDEL
QVQGNSLAKNVLSGGKGNDKLYGSEGADLLDGGEGNDLLKGGYGNDIYRYLSGYGHHIID
DEGGKDDKLSLADIDFRDVAFKREGNDLIMYKAEGNVLSIGHKNGITFKNWFEKESDDLS
NHQIEQIFDKDGRVITPDSLKKAFEYQQSNNKVSYVYGHDASTYGSQDNLNPLINEISKI
ISAAGNFDVKEERSAASLLQLSGNASDFSYGRNSITLTASA