Protein Info for EFB2_03971 in Escherichia fergusonii Becca

Annotation: 2-isopropylmalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 523 TIGR00973: 2-isopropylmalate synthase" amino acids 4 to 497 (494 residues), 861.9 bits, see alignment E=6.1e-264 PF00682: HMGL-like" amino acids 5 to 273 (269 residues), 360 bits, see alignment E=1.1e-111 PF22617: HCS_D2" amino acids 287 to 367 (81 residues), 80.7 bits, see alignment E=1.3e-26 PF08502: LeuA_dimer" amino acids 369 to 499 (131 residues), 133.2 bits, see alignment E=8.3e-43

Best Hits

Swiss-Prot: 100% identical to LEU1_ECO55: 2-isopropylmalate synthase (leuA) from Escherichia coli (strain 55989 / EAEC)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 100% identity to eco:b0074)

MetaCyc: 100% identical to 2-isopropylmalate synthase (Escherichia coli K-12 substr. MG1655)
2-isopropylmalate synthase. [EC: 2.3.3.13]

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (523 amino acids)

>EFB2_03971 2-isopropylmalate synthase (Escherichia fergusonii Becca)
MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQT
IARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIE
RAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAINAGATTINIPDTVGYTMPFEF
AGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAVHAGARQIEGAMNGIGERAGNCS
LEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQICNMPIPANKAIVGSGAFAHSSGIH
QDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGRAAVKHRMDEMGYKESEYNLDNLYDAF
LKLADKKGQVFDYDLEALAFIGKQQEEPEHFRLDYFSVQSGSNDIATAAVKLACGEEVKA
EAANGNGPVDAVYQAINRITDYNVELVKYSLTAKGHGKDALGQVDIVANYNGRRFHGVGL
ATDIVESSAKAMVHVLNNIWRAAEVEKELQRKAQHNENNKETV