Protein Info for EFB2_03741 in Escherichia fergusonii Becca

Annotation: Glyoxal reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 51% identical to GR_BACSU: Glyoxal reductase (yvgN) from Bacillus subtilis (strain 168)

KEGG orthology group: K06221, 2,5-diketo-D-gluconate reductase A [EC: 1.1.1.274] (inferred from 99% identity to eum:ECUMN_0336)

MetaCyc: 51% identical to glyoxal/methylglyoxal reductase (Bacillus subtilis subtilis 168)
Aryl-alcohol dehydrogenase (NADP(+)). [EC: 1.1.1.91]; 1.1.1.91 [EC: 1.1.1.91]; Methylglyoxal reductase (NADPH-dependent). [EC: 1.1.1.91, 1.1.1.283]

Predicted SEED Role

"oxidoreductase, aldo/keto reductase family"

MetaCyc Pathways

ketogluconate metabolism (8/8 steps found)
superpathway of methylglyoxal degradation (7/8 steps found)
methylglyoxal degradation IV (3/3 steps found)
methylglyoxal degradation V (3/3 steps found)
L-ascorbate biosynthesis VIII (engineered pathway) (4/7 steps found)

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.274

Use Curated BLAST to search for 1.1.1.274 or 1.1.1.283 or 1.1.1.91

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (283 amino acids)

>EFB2_03741 Glyoxal reductase (Escherichia fergusonii Becca)
MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLIDTAASYQNEIQVGNALK
QSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVDLYLIHQPYGDVHGAWRAM
EELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEVNPFNQQLQAVPWMQSRGIQP
EAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWIYQRGIVSLAKSVRKERMEENIN
ILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWLTGRKLDV