Protein Info for EFB2_03510 in Escherichia fergusonii Becca

Annotation: Enterobactin synthase component D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF17837: 4PPT_N
  • amino acids 78 to 141 (64 residues), 65.9 bits, E=2.7e-22
• PF01648: ACPS
  • amino acids 144 to 244 (101 residues), 52.8 bits, E=3.8e-18

Best Hits

Swiss-Prot: 96% identical to ENTD_ECOLI: Enterobactin synthase component D (entD) from Escherichia coli (strain K12)

KEGG orthology group: K02362, enterobactin synthetase component D [EC: 2.7.8.-] (inferred from 100% identity to ecc:c0668)

MetaCyc: 96% identical to phosphopantetheinyl transferase EntD (Escherichia coli K-12 substr. MG1655)
2,3-dihydroxybenzoate--serine ligase. [EC: 6.3.2.14]; Holo-[acyl-carrier-protein] synthase. [EC: 6.3.2.14, 2.7.8.7]; 2.7.8.7 [EC: 6.3.2.14, 2.7.8.7]

Predicted SEED Role

"4'-phosphopantetheinyl transferase (EC 2.7.8.-) [enterobactin] siderophore" in subsystem Siderophore Enterobactin (EC 2.7.8.-)

MetaCyc Pathways

• superpathway of chorismate metabolism (54/59 steps found)
• acyl carrier protein metabolism (2/2 steps found)
• acyl carrier protein activation (1/1 steps found)
• enterobactin biosynthesis (7/11 steps found)
• colibactin biosynthesis (26/38 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (3/8 steps found)
• petrobactin biosynthesis (1/10 steps found)

KEGG Metabolic Maps

• Aminophosphonate metabolism
• Glycerophospholipid metabolism
• High-mannose type N-glycan biosynthesis
• Nucleotide sugars metabolism
• Pantothenate and CoA biosynthesis
• Sphingolipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.8.-, 2.7.8.7

Use Curated BLAST to search for 2.7.8.- or 2.7.8.7 or 6.3.2.14

Protein Sequence (247 amino acids)

>EFB2_03510 Enterobactin synthase component D (Escherichia fergusonii Becca)
MNALSGLQKSCQFNILQDHVGLISVAHQAVLRLSSFSNMVDMKTTHTSLPFAGHTLHFVE
FDPASFREQDLLWLPHYAQLQHAGRKRKTEHLAGRIAAIYALREYGYKCVPAIGELRQPV
WPAGVYGSISHCGTTALAVVSRQPIGIDIEEIFSAQTARELTDNIITPAEHKRLADCGLA
FPLALTLAFSAKESAFKASEIQTDADFLDYQIISRNKQQVIIHRENEMFAVHWQIKEKIV
ITLCQHD