Protein Info for EFB2_03424 in Escherichia fergusonii Becca

Annotation: PTS system N-acetylglucosamine-specific EIICBA component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 648 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 37 to 61 (25 residues), see Phobius details amino acids 69 to 88 (20 residues), see Phobius details amino acids 94 to 112 (19 residues), see Phobius details amino acids 132 to 153 (22 residues), see Phobius details amino acids 165 to 184 (20 residues), see Phobius details amino acids 193 to 212 (20 residues), see Phobius details amino acids 228 to 252 (25 residues), see Phobius details amino acids 259 to 276 (18 residues), see Phobius details amino acids 282 to 328 (47 residues), see Phobius details amino acids 337 to 359 (23 residues), see Phobius details TIGR01998: PTS system, N-acetylglucosamine-specific IIBC component" amino acids 3 to 463 (461 residues), 713.3 bits, see alignment E=2.1e-218 PF02378: PTS_EIIC" amino acids 12 to 300 (289 residues), 248.5 bits, see alignment E=1.3e-77 TIGR00826: PTS system, glucose-like IIB component" amino acids 360 to 452 (93 residues), 90.3 bits, see alignment E=1.2e-29 PF00367: PTS_EIIB" amino acids 395 to 427 (33 residues), 46.8 bits, see alignment (E = 2.3e-16) PF00358: PTS_EIIA_1" amino acids 500 to 624 (125 residues), 157.5 bits, see alignment E=2.1e-50 TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 501 to 621 (121 residues), 158.3 bits, see alignment E=1.2e-50

Best Hits

Swiss-Prot: 99% identical to PTW3C_ECOLI: PTS system N-acetylglucosamine-specific EIICBA component (nagE) from Escherichia coli (strain K12)

KEGG orthology group: K02802, PTS system, N-acetylglucosamine-specific IIA component [EC: 2.7.1.69] K02803, PTS system, N-acetylglucosamine-specific IIB component [EC: 2.7.1.69] K02804, PTS system, N-acetylglucosamine-specific IIC component (inferred from 100% identity to ecv:APECO1_1393)

MetaCyc: 99% identical to N-acetylglucosamine-specific PTS enzyme IIABC component (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-167 [EC: 2.7.1.193]; TRANS-RXN-167A [EC: 2.7.1.193, 2.7.1.191]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.191 or 2.7.1.193 or 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (648 amino acids)

>EFB2_03424 PTS system N-acetylglucosamine-specific EIICBA component (Escherichia fergusonii Becca)
MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQAGGAIFDNLALIFAIGV
ASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGIITGLVGGAAYNRWSDIKL
PDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIHAGGEWIVSAGALGSGIFGFI
NRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINRFYAGDGTAGMFMSGFFPIMMFG
LPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGVTEPLEFLFMFLAPLLYLLHALLTG
ISLFVATLLGIHAGFSFSAGAIDYALMYNLPAASQNVWMLLVMGVVFFAIYFVVFSLVIR
MFNLKTPGREDKEDEIVTEEANSNTEEGLNQLATNYIAAVGGTDNLKAIDACITRLRLTV
VDSARVNDAMCKRLGASGVVKLNKQTIQVIVGAKAESIGDAMKKVVARGPVAAASAEATP
ATAAPVAKPQAVPNAVSIAELVSPITGDVVALDQVPDEAFASKAVGDGVAVKPTDKIVVS
PAAGTIVKIFNTNHAFCLETEKGAEIVVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILE
MDLDYLNANARSMISPVVCSNIDDFSGLIIKAQGHVVAGQTPLYEIKK