Protein Info for EFB2_03249 in Escherichia fergusonii Becca

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 804 PF05840: Phage_GPA" amino acids 188 to 510 (323 residues), 425.6 bits, see alignment E=5.7e-132

Best Hits

Swiss-Prot: 98% identical to ENDPH_SHIBS: Probable replication endonuclease from prophage-like region (SBO_0752) from Shigella boydii serotype 4 (strain Sb227)

KEGG orthology group: None (inferred from 99% identity to ecx:EcHS_A0918)

Predicted SEED Role

"Phage replication protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (804 amino acids)

>EFB2_03249 hypothetical protein (Escherichia fergusonii Becca)
MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSTLSNLITLYAADNEQEQLR
REALSDQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQRFNSDMVILADVNALPSHI
SKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRDSQLSASFRFMASHEGLDGLL
ILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDVKPEEIRKTWEKVAAETLRLDVI
PPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWYRKLWKMRCEWREEQLRAVCLVSKK
ASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDGDTLDMEDVVNASSSNPAHRRNEMMAC
VKGLELIAEMRGDCAVFYTITCPSRFHSTLNNGRPNPTWTNATVRQSSDYLVGMFAAFRK
AMHKAGLRWYGVRVAEPHHDGTVHWHLLCFMRKKDRRAITALLRKFAIREDREELGNNTG
PRFKSELINPRKGTPTSYIAKYISKNIDGRGLAGEISKETGKSLRDNAEYVNAWASLHRV
QQFRFFGIPGRQAYRELRLLAGQAARQQGDKKAGAPVLDNPRLDAILAAADAGCFATYIM
KQGGVLVPRKYHLIRTAYEINEEPTAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAV
DVQEAAADQGACAPWTRGNNCPLAENLNQQEKDKSADGDIRTDITRMDDKELHDYLHSMS
KKERRELAARLRLVKPKRRKDYKQRITDHQRLQLVYELKSRGFDGSEKEVDLLLRGGSIP
SGAGLRIFYRNQRLQEDDKWRNLY