Protein Info for EFB2_03066 in Escherichia fergusonii Becca

Annotation: Bifunctional protein PutA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21775: PutA_1st
  • amino acids 11 to 43 (33 residues), 75.1 bits, see alignment (E = 5.8e-25)
• PF18327: PRODH
  • amino acids 89 to 136 (48 residues), 64.6 bits, see alignment (E = 2e-21)
• PF14850: Pro_dh-DNA_bdg
  • amino acids 148 to 259 (112 residues), 155.2 bits, E=2.1e-49
• PF01619: Pro_dh
  • amino acids 268 to 569 (302 residues), 360.7 bits, E=1.5e-111
• TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase
  • amino acids 610 to 1112 (503 residues), 871 bits, E=1.1e-266
• PF00171: Aldedh
  • amino acids 659 to 1103 (445 residues), 368.3 bits, E=1.3e-113

Best Hits

Swiss-Prot: 99% identical to PUTA_ECOLI: Bifunctional protein PutA (putA) from Escherichia coli (strain K12)

MetaCyc: 99% identical to fused DNA-binding transcriptional repressor / proline dehydrogenase / 1-pyrroline-5-carboxylate dehydrogenase PutA (Escherichia coli K-12 substr. MG1655)
RXN0-7008 [EC: 1.5.5.2]; RXN-14116 [EC: 1.5.5.2, 1.2.1.88]

Predicted SEED Role

No annotation

MetaCyc Pathways

• L-proline degradation I (2/3 steps found)
• proline to cytochrome bo oxidase electron transfer (1/2 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• L-arginine degradation I (arginase pathway) (1/3 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)
• ethene biosynthesis II (microbes) (1/4 steps found)
• L-N^δ-acetylornithine biosynthesis (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.88 or 1.5.5.2

Protein Sequence (1320 amino acids)

>EFB2_03066 Bifunctional protein PutA (Escherichia fergusonii Becca)
MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQLENSDTLPELPALLSGA
ANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRPETEAVSMLLEQARLPQPV
AEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQEGVALMCLAEALLRIPDKAT
RDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGKLVSTHNEASLSRSLNRIIGKSG
EPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLEEKGFRYSYDMLGEAALTAADAQAY
MVSYQQAIHAIGKASNGRGIYEGPGISIKLSALHPRYSRAQYDRVMEELYPRLKSLTLLA
RQYDIGINIDAEEADRLEISLDLLEKLCFEPELAGWNGIGFVIQAYQKRCPLVIDYLIDL
ATRSRRRLMIRLVKGAYWDSEIKRAQMDGLEGYPVYTRKVYTDVSYLACAKKLLAVPNLI
YPQFATHNAHTLAAIYQLAGQNYYPGQYEFQCLHGMGEPLYEQVTGKVADGKLNRPCRIY
APVGTHETLLAYLVRRLLENGANTSFVNRIADTSLPLDELVADPVTAVEKLAQQEGQTGL
PHPKIPLPRDLYGHGRDNSAGLDLANEHRLASLSSALLNSALQKWQSLPMLEQSVAAGEM
SPVINPAEPKDIVGYVREATPREVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQ
MQQLIGILVREAGKTFSNAIAEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNF
PLAIFTGQIAAALAAGNSVLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGA
QLTGDDRVRGVMFTGSTEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQ
VVIDVLASAFDSAGQRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVI
DSEAKANIERHIQTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVF
GPVLHVVRYNRNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAQVGNLYVNRNMVG
AVVGVQPFGGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALT
QPLNALREWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDE
QDALTQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHG
DSDQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG