Protein Info for EFB2_02924 in Escherichia fergusonii Becca

Annotation: Glucans biosynthesis glucosyltransferase H

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 837 transmembrane" amino acids 129 to 146 (18 residues), see Phobius details amino acids 182 to 207 (26 residues), see Phobius details amino acids 502 to 527 (26 residues), see Phobius details amino acids 556 to 580 (25 residues), see Phobius details amino acids 589 to 607 (19 residues), see Phobius details amino acids 613 to 630 (18 residues), see Phobius details amino acids 650 to 666 (17 residues), see Phobius details amino acids 672 to 696 (25 residues), see Phobius details PF00535: Glycos_transf_2" amino acids 236 to 418 (183 residues), 72.4 bits, see alignment E=6.8e-24 PF13632: Glyco_trans_2_3" amino acids 332 to 534 (203 residues), 56.6 bits, see alignment E=5.2e-19

Best Hits

Swiss-Prot: 100% identical to OPGH_ECOL6: Glucans biosynthesis glucosyltransferase H (mdoH) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K03669, membrane glycosyltransferase [EC: 2.4.1.-] (inferred from 100% identity to ecv:APECO1_134)

Predicted SEED Role

"Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-)" (EC 2.4.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.-

Use Curated BLAST to search for 2.4.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (837 amino acids)

>EFB2_02924 Glucans biosynthesis glucosyltransferase H (Escherichia fergusonii Becca)
MPIAASEKAALPKTDIRAVHQALDAEHRTWAREDDSPQGSVKARLEQAWPDSLADGQLIK
DDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRFWDRLRGRDVTPRYLARLTKEEQESEQKW
RTVGTIRRYILLILTLAQTVVATWYMKTILPYQGWALINPMDMVGQDVWVSFMQLLPYML
QTGILILFAVLFCWVSAGFWTALMGFLQLLIGRDKYSISASTVGDEPLNPEHRTALIMPI
CNEDVNRVFAGLRATWESVKATGNAKHFDVYILSDSYNPDICVAEQKAWMELIAEVGGEG
QIFYRRRRRRVKRKSGNIDDFCRRWGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAG
IIQSSPKASGMDTLYARCQQFATRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHC
ALAPLPGEGSFAGSILSHDFVEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRW
CHGNLMNFRLFLVKGMHPVHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFL
QPRQLFPVWPQWRPELAIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLE
VLFSVLLAPVRMLFHTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWA
VGMAWLDLRFLFWLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVD
TDRFLEMNRQRSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETP
EKLNRDRRLVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ