Protein Info for EFB2_02526 in Escherichia fergusonii Becca

Annotation: Respiratory nitrate reductase 2 gamma chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 226 transmembrane" amino acids 12 to 29 (18 residues), see Phobius details amino acids 50 to 70 (21 residues), see Phobius details amino acids 90 to 111 (22 residues), see Phobius details amino acids 126 to 149 (24 residues), see Phobius details amino acids 183 to 204 (22 residues), see Phobius details TIGR00351: respiratory nitrate reductase, gamma subunit" amino acids 2 to 226 (225 residues), 414.3 bits, see alignment E=7.2e-129 PF02665: Nitrate_red_gam" amino acids 6 to 225 (220 residues), 252.2 bits, see alignment E=2.2e-79

Best Hits

Swiss-Prot: 88% identical to NARV_ECO57: Respiratory nitrate reductase 2 gamma chain (narV) from Escherichia coli O157:H7

KEGG orthology group: K08347, nitrate reductase 2, gamma subunit [EC: 1.7.99.4] (inferred from 98% identity to ecm:EcSMS35_1709)

MetaCyc: 88% identical to nitrate reductase Z subunit gamma (Escherichia coli K-12 substr. MG1655)
1.97.1.-; RXN0-3501 [EC: 1.7.5.1]

Predicted SEED Role

"Respiratory nitrate reductase gamma chain (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.5.1 or 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (226 amino acids)

>EFB2_02526 Respiratory nitrate reductase 2 gamma chain (Escherichia fergusonii Becca)
MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQMLDKRGMVLWSNLFHIGI
LGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLTLIGGAGLLVRRLTNPRIR
ATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKLVGWAQSIVTFQGGASTHLDG
VAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRRYQIVRSRR