Protein Info for EFB2_02443 in Escherichia fergusonii Becca

Annotation: Serine/threonine-protein kinase toxin HipA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 TIGR03071: HipA N-terminal domain" amino acids 4 to 103 (100 residues), 103.8 bits, see alignment E=2.3e-34 PF13657: Couple_hipA" amino acids 6 to 103 (98 residues), 95 bits, see alignment E=3.3e-31 PF07804: HipA_C" amino acids 149 to 401 (253 residues), 177.2 bits, see alignment E=3.8e-56

Best Hits

Swiss-Prot: 98% identical to HIPA_ECOLI: Serine/threonine-protein kinase toxin HipA (hipA) from Escherichia coli (strain K12)

KEGG orthology group: K07154, (no description) (inferred from 100% identity to ecz:ECS88_1594)

MetaCyc: 98% identical to serine/threonine-protein kinase toxin HipA (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"HipA protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (440 amino acids)

>EFB2_02443 Serine/threonine-protein kinase toxin HipA (Escherichia fergusonii Becca)
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNF
FDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKL
TEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTALLRIGNDWCIPKGITPTTHII
KLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQV
FQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASK
GKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIPAALDNVKTSLPTDFPENVVT
AVETNVLRLHGRLSREYGIK