Protein Info for EFB2_01916 in Escherichia fergusonii Becca

Annotation: Multidrug export protein MepA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 479 transmembrane" amino acids 32 to 56 (25 residues), see Phobius details amino acids 67 to 90 (24 residues), see Phobius details amino acids 111 to 134 (24 residues), see Phobius details amino acids 154 to 175 (22 residues), see Phobius details amino acids 187 to 206 (20 residues), see Phobius details amino acids 218 to 244 (27 residues), see Phobius details amino acids 257 to 280 (24 residues), see Phobius details amino acids 300 to 320 (21 residues), see Phobius details amino acids 332 to 358 (27 residues), see Phobius details amino acids 374 to 393 (20 residues), see Phobius details amino acids 404 to 428 (25 residues), see Phobius details amino acids 434 to 454 (21 residues), see Phobius details TIGR00797: MATE efflux family protein" amino acids 38 to 435 (398 residues), 168.2 bits, see alignment E=1.3e-53 PF01554: MatE" amino acids 38 to 201 (164 residues), 81.4 bits, see alignment E=3e-27 amino acids 264 to 423 (160 residues), 59.6 bits, see alignment E=1.5e-20

Best Hits

KEGG orthology group: None (inferred from 100% identity to cko:CKO_00872)

MetaCyc: 100% identical to precolibactin exporter ClbM (Escherichia coli IHE3034)
TRANS-RXN-393

Predicted SEED Role

"Putative inner membrane protein"

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (479 amino acids)

>EFB2_01916 Multidrug export protein MepA (Escherichia fergusonii Becca)
MTVNKLEPDNGTPNDELFTVAGMFDGSLYKLLLRMALPMFVGMLTQVTYAIADIFWLSHI
DVTNSGIIAGVGLVFPVGMGLFAIANGIQIGMGSLLSRAIGMQRLDRAQRILSVGIIIAL
FFAIVITVLGYVYAQPLLRSLGATKSIIGYATEFYYYSLLTVFSIMLIGVMMGLFQGAGK
IMVIMKASLLGALVNIMLDPIMIFVFDFGVKGVALASFLAQLSMVAYFIYTLMGLHIGLS
IRIALRPFSWKIYREFLSVGMAQMLMQLIIAVGIVIYNFFIVRLDVNAMAAFTLTGRIDY
FIITPMLAIATALLTVVGQNWGHGNVTRTLNAYWAAVALAFSIVLVLAVMHIVLAPWMYP
LFTRVVAVSDYAVLQTRIMALALPFVAISLLASEYYQAIGKPWYSVLLTLMRHVFISVPV
VYLLAIVLEMRITGVYFGAMSGTFVAALLAWRLLRLSPRLLRWNQEAVRSQHLDMEVAP