Protein Info for EFB2_01911 in Escherichia fergusonii Becca

Annotation: Gramicidin S synthase 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1500 1598 transmembrane" amino acids 76 to 96 (21 residues), see Phobius details amino acids 211 to 225 (15 residues), see Phobius details amino acids 458 to 476 (19 residues), see Phobius details amino acids 773 to 786 (14 residues), see Phobius details amino acids 1038 to 1062 (25 residues), see Phobius details amino acids 1177 to 1198 (22 residues), see Phobius details amino acids 1447 to 1457 (11 residues), see Phobius details PF00501: AMP-binding" amino acids 25 to 368 (344 residues), 272.3 bits, see alignment E=1.2e-84 amino acids 994 to 1336 (343 residues), 227.8 bits, see alignment E=4.1e-71 TIGR01733: amino acid adenylation domain" amino acids 42 to 446 (405 residues), 387.6 bits, see alignment E=3.3e-120 amino acids 1011 to 1408 (398 residues), 326 bits, see alignment E=1.6e-101 PF13193: AMP-binding_C" amino acids 430 to 503 (74 residues), 39.5 bits, see alignment 1.8e-13 PF00668: Condensation" amino acids 532 to 967 (436 residues), 299.2 bits, see alignment E=1e-92 PF00550: PP-binding" amino acids 1496 to 1560 (65 residues), 48 bits, see alignment (E = 2.4e-16)

Best Hits

KEGG orthology group: None (inferred from 100% identity to cko:CKO_00867)

MetaCyc: 100% identical to colibactin non-ribosomal peptide synthetase ClbH (Escherichia coli IHE3034)
6.2.1.M38 [EC: 6.2.1.M38]; RXN-16906 [EC: 6.2.1.M38, 6.2.1.72]; RXN-21112 [EC: 6.2.1.M38, 6.2.1.72]

Predicted SEED Role

No annotation

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.72 or 6.2.1.M38

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1598 amino acids)

>EFB2_01911 Gramicidin S synthase 2 (Escherichia fergusonii Becca)
MEQQGIMRQLPTDDQTIVDYLYRIAGEYGEKAAVLMGDAALSYHDLNARSNQLAHYLRGL
GIGEDRVVAIRLPRGMAMLIAIFAIVKAGGAYLPLAYNAPRSRIENILSNSGAVCLIGTD
DGDRWPIPRVEIDSAAVSAMPTTDLRYRPHARQLAYIIYTSGSTGVPKGVATEHAALLNR
IVWMQNAYPISSQDVLFQKTVYTFDVSVWEMFWWAMYGASVVLLPSGLESDPRTLARLIQ
RHRVSVVHFVPSMLNLFVEYLEMKQDPRLTASLRLVFSSGEKLTVHSVARFYQSVAQGDL
INLYGPTEAAIDVSHHRCLRGYDYDDIPIGQAIDGCRLYVLDDHGNPVADGEEGELYLAG
IGLARGYLNNVALTDRCFTIHPTLRHLGKPERLYKTGDLVWRDGESQQIHYIGRNDFQIK
IRGLRVELGEIEAHAMRFPGVQQAVVVADQDDPDNQLIYAFVVSSVPLNLAALMDALSKN
LPAYMLPNRLLAMSELPLSDNGKCCRKTLLDLARAYSASRVDLRETPAVRYLPLSSAQSS
MWFMQQLAPHTALYNNPTALLLEGELDRTRMDGAIRQLMSRHTLLRAMAETHNGQPVLAV
PQCVSSQALLTIVPLPSVSDDNALQAMINQRAAHPMPLTSGTPLCRFELLTLDDDRSVLL
IHLHHIISDGWSKGVLLRELQAAYNGESLTPEPLLEYADYMEYQEEWRQSDAYQDAMRYW
QNTLAGTLPILDIPTDQPRQKVARYQGAFVAFALSANTCERVLAAARAQRVSLYNYLLTA
FVLLLHRNARQQEYIVGMPIAARLTKEQEHMIAPLVNVLPLRLPLDEAASFSELVQTIRG
ILFAAFRHQRLEFTDIVRAVNVDRSAGHFPIYQCMFQLDNMPLASPTLNGVNVTPLLLDT
SASQVDISLSMQHIDGRITGTFEYDAGLYSADRIQHLVAQWRELLDEASSQPTQLVRDLI
RFTPREHAWLARHNATEVALPPVDNLLALVLPHCQQRPTQVALRHADDAMTYGELQQATM
QMCTWLRAQGVKRGESVALQLPFCFELIIAQLAILSLGASYVPLDGNAPAARNALILAQA
TPCMLLVAQPLESPHGLTIPWVLVPDWRSLLTEIPNLPVSVAPDALDCDAVVIFTSGTTG
QPKGVRLSQRNLVNLTASFISSYQVTHQDVLLPITSVASASFVGEVLPLLAAGGTLVLAQ
KAQSLDSDALIALLASQRVTILSTTPSLSASLSVLAQSMGSLRLFLCGGEALEYEQIAPL
LPHMAVVNGYGLTESGICSTYFPVAKRREQETGALPIGRPIQNTQAYVVDAYNRLVPPGA
CGELCFSGLGISPGYLDARQDPERFVELPEYPGVRVLKTGDRARWATDGMLFYLGRQDRQ
VQIRGYRVELGDIESLLKQHPDIADAWVDVRRNAAATPLLVAFYCSVNGVALDAQQLRVW
LSLRLPLHMLPLLYVPLSAMPLGVNGKIDPQCLPLVDLRQLEGPGEYVPPATELEQRLAE
IWQQLLGLERVGTTTNFFDLGGHSLLLVQMQQYIGQQCGQHVALVDLLRFTTIKRLAEFL
LAPDAAQGTTGDQTQLRAAKQRLAFGHTRWAATTDSHH