Protein Info for EFB2_01690 in Escherichia fergusonii Becca

Annotation: PTS system fructose-specific EIIB'BC component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 563 transmembrane" amino acids 234 to 256 (23 residues), see Phobius details amino acids 266 to 291 (26 residues), see Phobius details amino acids 303 to 329 (27 residues), see Phobius details amino acids 349 to 369 (21 residues), see Phobius details amino acids 381 to 403 (23 residues), see Phobius details amino acids 427 to 451 (25 residues), see Phobius details amino acids 463 to 482 (20 residues), see Phobius details amino acids 489 to 511 (23 residues), see Phobius details amino acids 519 to 551 (33 residues), see Phobius details PF02302: PTS_IIB" amino acids 107 to 196 (90 residues), 74.4 bits, see alignment E=9.8e-25 TIGR00829: PTS system, Fru family, IIB component" amino acids 107 to 190 (84 residues), 133.2 bits, see alignment E=3.2e-43 TIGR01427: PTS system, Fru family, IIC component" amino acids 213 to 552 (340 residues), 500.2 bits, see alignment E=3.6e-154 PF02378: PTS_EIIC" amino acids 233 to 496 (264 residues), 75 bits, see alignment E=6.1e-25

Best Hits

Swiss-Prot: 98% identical to PTFBC_ECOLI: PTS system fructose-specific EIIB'BC component (fruA) from Escherichia coli (strain K12)

KEGG orthology group: K02769, PTS system, fructose-specific IIB component [EC: 2.7.1.69] K02770, PTS system, fructose-specific IIC component (inferred from 100% identity to ecz:ECS88_2315)

MetaCyc: 98% identical to fructose-specific PTS multiphosphoryl transfer protein FruA (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69)" in subsystem Fructose utilization (EC 2.7.1.69)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (563 amino acids)

>EFB2_01690 PTS system fructose-specific EIIB'BC component (Escherichia fergusonii Becca)
MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNEAEMAIVLGDSIPNDSALNGK
NVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASGPKRVVAVTACPTGVAHTF
MAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAADLVIVAADIEVDLAKFAGKPM
YRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTEGKKESAGAYRHLLTGVSYMLPM
VVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGSAFALMVPVLAGYIAFSIADRPGLT
PGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLISTQLKLPQSMEALKPILIIPLISSLVV
GLAMIYLIGKPVAGILDGLTHWLQTMGTANAVLLGAILGGMMCTDMGGPVNKAAYAFGVG
LLSTQTYGPMAAIMAAGMVPPLAMGLATMVARRKFDKAQQEGGKAALVLGLCFISEGAIP
FAARDPMRVLPCCIVGGALTGAISMAIGAKLMAPHGGLFVLLIPGAITPVLGYLVAIIAG
TLVAGLAYAFLKRPETQIVEKNA