Protein Info for EFB2_01615 in Escherichia fergusonii Becca
Annotation: Anaerobic glycerol-3-phosphate dehydrogenase subunit B
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to GLPB_ECO81: Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) from Escherichia coli O81 (strain ED1a)
KEGG orthology group: K00112, glycerol-3-phosphate dehydrogenase subunit B [EC: 1.1.5.3] (inferred from 98% identity to eco:b2242)MetaCyc: 98% identical to anaerobic glycerol-3-phosphate dehydrogenase subunit B (Escherichia coli K-12 substr. MG1655)
Glycerol-3-phosphate dehydrogenase. [EC: 1.1.5.3]
Predicted SEED Role
"Anaerobic glycerol-3-phosphate dehydrogenase subunit B (EC 1.1.5.3)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Respiratory dehydrogenases 1 (EC 1.1.5.3)
MetaCyc Pathways
- glycerol and glycerophosphodiester degradation (4/4 steps found)
- sn-glycerol 3-phosphate anaerobic respiration (3/3 steps found)
- glycerol degradation I (3/3 steps found)
- glycerol-3-phosphate shuttle (2/2 steps found)
- glycerol-3-phosphate to fumarate electron transfer (2/2 steps found)
- glycerol-3-phosphate to hydrogen peroxide electron transport (2/2 steps found)
- glycerophosphodiester degradation (2/2 steps found)
- nitrate reduction IX (dissimilatory) (2/2 steps found)
- glycerol 3-phosphate to cytochrome aa3 oxidase electron transfer (1/2 steps found)
- glycerol-3-phosphate to cytochrome bo oxidase electron transfer (1/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.5.3
Use Curated BLAST to search for 1.1.5.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (419 amino acids)
>EFB2_01615 Anaerobic glycerol-3-phosphate dehydrogenase subunit B (Escherichia fergusonii Becca) MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGSLDLLSHLPDGQPVTEI HSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQLQGSVELAHQRITPLGTLR STWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLRELDLKVETAEIELPELDVLR NNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMILMPACFGLADDKLWHWLNEKLP CSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPGDEVKKVTCKNGVVNEIWTRNHADI PLRPRFAVLASGSFFSGGLVAERDGIREPILGLDVLQTATRGEWYKGDFFAPQPWQQFGV TTDEALRPSQAGQTIENLFAIGSVLGGFDPIAQGCGGGVCAVSALHVAQQIAQRAGGQQ