Protein Info for EFB2_01605 in Escherichia fergusonii Becca

Annotation: Nucleoside triphosphatase NudI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00293: NUDIX
  • amino acids 1 to 136 (136 residues), 82.1 bits, E=1.9e-27

Best Hits

Swiss-Prot: 100% identical to NUDI_ECOUT: Nucleoside triphosphatase NudI (nudI) from Escherichia coli (strain UTI89 / UPEC)

KEGG orthology group: K12944, nucleoside triphosphatase [EC: 3.6.1.-] (inferred from 99% identity to eco:b2251)

MetaCyc: 99% identical to pyrimidine deoxynucleotide diphosphatase NudI (Escherichia coli K-12 substr. MG1655)
dCTP diphosphatase. [EC: 3.6.1.12, 3.6.1.65, 3.6.1.9]; 3.6.1.9 [EC: 3.6.1.12, 3.6.1.65, 3.6.1.9]; RXN-17104 [EC: 3.6.1.12, 3.6.1.65, 3.6.1.9, 3.6.1.68]; dUTP diphosphatase. [EC: 3.6.1.12, 3.6.1.65, 3.6.1.9, 3.6.1.68, 3.6.1.23]

Predicted SEED Role

"Pyrimidine deoxynucleoside triphosphate (dYTP) pyrophosphohydrolase YfoO" in subsystem Nudix proteins (nucleoside triphosphate hydrolases)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (7/7 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides dephosphorylation (2/3 steps found)
• geraniol biosynthesis (cytosol) (1/2 steps found)
• dZTP biosynthesis (3/5 steps found)
• juvenile hormone III biosynthesis I (1/6 steps found)
• tunicamycin biosynthesis (2/9 steps found)

KEGG Metabolic Maps

• Folate biosynthesis
• Lipopolysaccharide biosynthesis
• Nicotinate and nicotinamide metabolism
• Pantothenate and CoA biosynthesis
• Purine metabolism
• Pyrimidine metabolism
• Riboflavin metabolism
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.1.23

Use Curated BLAST to search for 3.6.1.- or 3.6.1.12 or 3.6.1.23 or 3.6.1.65 or 3.6.1.68 or 3.6.1.9

Protein Sequence (141 amino acids)

>EFB2_01605 Nucleoside triphosphatase NudI (Escherichia fergusonii Becca)
MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGERIEEALRREIREELGE
QLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANREVKINEEFQDYAWVKPE
DLVHYDLNVATRKTLRLKGLL